{ "@context": "https://schema.org", "@type": "Product", "name": "2xYT Agar Powder – Rich Microbiological Culture Medium", "description": "2xYT Agar is a high-nutrient microbiological medium designed for E. coli growth, recombinant DNA cloning, and M13 phage propagation in molecular biology research.", "brand": { "@type": "Brand", "name": "Biofargo" }, "keywords": "2xYT agar, 2xYT medium, E. coli culture medium, M13 phage agar, rich bacterial growth medium, tryptone yeast extract agar, microbiological media powder" } Description In molecular biology and recombinant protein research, inconsistent bacterial growth, low phage yield, and poor plasmid amplification are often caused by nutrient-limited or low-quality culture media. Researchers working with E. coli expression systems and filamentous phage display require a richer and more stable alternative to standard LB medium. 2×YT Agar (2xYT Medium) is a nutritionally enriched microbiological growth medium optimized for high-density E. coli cultivation and efficient filamentous phage (M13) propagation. Compared with standard LB medium, 2×YT provides higher levels of peptides, amino acids, vitamins, and nitrogen sources, resulting in improved cell density and plasmid yield. This dehydrated agar formulation combines tryptone, yeast extract, sodium chloride, and agar to support robust bacterial metabolism and stable solid-phase culture performance. It is widely used in cloning workflows, phage display systems, recombinant DNA amplification, and protein expression studies. Specification Total Product Size 250 g Container Size 250 g Number of Containers 1 Form Dehydrated powder medium Application E. coli culture / Phage propagation / Cloning Shipping Condition Room Temperature (RT) UNSPSC Code 41106200 Category Microbiological Culture Media Features High-nutrient enriched formulation for enhanced E. coli growth and metabolic activity. Optimized for M13 filamentous phage replication and phage display systems. Supports high-efficiency plasmid amplification and recombinant DNA cloning workflows. Rich tryptone and yeast extract composition for improved bacterial density and protein yield. Stable agar-based solid medium for consistent plate preparation and reproducible results. Applications Recombinant DNA cloning and plasmid amplification in E. coli. M13 filamentous phage propagation and phage display research. High-density bacterial culture for protein expression systems. Microbiological solid media preparation for laboratory research. Molecular biology workflows requiring rich bacterial growth medium. Genetic engineering and synthetic biology experiments. Composition (per liter) Tryptone: 16.0 g Yeast Extract: 10.0 g Sodium Chloride: 5.0 g Agar: 15.0 g Preparation Instructions Dissolve 46 g of 2xYT Agar powder in 1 L distilled water. Heat with stirring until fully dissolved and boiling. Autoclave at 121°C for 15 minutes for sterilization. Pour into sterile Petri dishes under aseptic conditions. pH: 6.8 ± 0.2 at 25°C Documents COA Frequently Asked Questions What is 2xYT Agar used for? It is a rich microbiological culture medium used for high-density growth of E. coli and propagation of M13 bacteriophage in molecular biology and cloning workflows. How is 2xYT different from LB medium? 2xYT contains higher concentrations of tryptone and yeast extract, providing more nutrients for improved bacterial growth, higher plasmid yield, and better phage production compared to LB. Why is 2xYT preferred for phage display? Its enriched nutrient composition supports efficient M13 phage replication and higher infection efficiency in E. coli host strains. Can 2xYT Agar be used for recombinant protein expression? Yes, it is widely used in recombinant protein expression systems due to its ability to support high cell density cultures. How should 2xYT Agar be stored? Store the dehydrated powder in a dry, tightly sealed container at room temperature away from moisture. 2xYT agar, 2xYT medium, rich bacterial growth medium, E. coli culture medium, M13 phage agar, tryptone yeast extract agar, LB alternative enriched medium, microbiology culture plates, recombinant DNA cloning medium, phage display medium
