Description 3-D Life CD-HyLink is a component of the 3-D Life Hydrogel system. CD-HyLink consists of hyaluronic acid (hyaluronan) carrying thiol groups along its backbone. When combined with SG-Dextran of the 3-D Life Hydrogel system, thiol groups on CD-HyLink form stable thioether bonds with the thiol-reactive groups on the polymer, which results in the formation of the hydrogel. CD-HyLink contains a matrix metalloproteinase (MMP)-cleavable peptide sequence (Pro-Leu-Gly-Leu-Trp-Ala), which is cleaved by a broad range of MMPs including MMP1, MMP2, MMP3, MMP7, MMP9 and MMP13. It allows cells to locally degrade the hydrogel. Note: Dextran hydrogels crosslinked with CD-HyLink cannot be dissolved by the addition of dextranase (3-D Life Dextranase, Cat. No. D10-1). For using this product please consult General Protocol GP-4B "Preparation of 3-D Life Hyaluronic Acid (CD-HA) Hydrogels containing a matrix metalloproteinase cleavage site" and the 3-D Life Hydrogels User Guide, which may be downloaded below. Application 3-D Life CD-HyLink is used together with thiol-reactive SG-Dextran of the 3-D Life Hydrogel system to establish 3D cell cultures. Quantity Cat. No. L80-1: Sufficient for 1.6 ml hydrogel of a crosslinking strength of 1.2 mmol/l when used with SG-Dextran of the 3-D Life Hydrogel system. Cat. No. L80-3: Sufficient for 4.8 ml hydrogel of a crosslinking strength of 1.2 mmol/l when used with SG-Dextran of the 3-D Life Hydrogel system. Components Cat. No. L80-1: Material Quantity Concentration of reactive groups CD-HyLink, Lyophilized 200 µl* 10 mmol/L* MSDS Water 600 µl n/a MSDS *Volume after reconstitution of lyophilizate. *Concentration after reconstitution of lyophilizate. Cat. No. L80-3: Material Quantity Concentration of reactive groups CD-HyLink, Lyophilized 3 x 200 µl* 10 mmol/L* MSDS Water 600 µl n/a MSDS *Volume after reconstitution of lyophilizate. *Concentration after reconstitution of lyophilizate. Resources Product Datasheet (PDS): 3-D Life CD-HyLink (200 µl) Product Datasheet (PDS): 3-D Life CD-HyLink (3 x 200 µl) 3-D Life User Guide & Protocols 3-D Life Biomimetic Hydrogels Page 3-D Life Hydrogels User Applications 3-D Life Hydrogels Product References Product References Kromidas, Elena et al. “Immunocompetent PDMS-Free Organ-on-Chip Model of Cervical Cancer Integrating Patient-Specific Cervical Fibroblasts and Neutrophils.” Advanced healthcare materials vol. 13,21 (2024): e2302714. doi:10.1002/adhm.202302714 Ilex Life Sciences LLC is an authorized distributor of Cellendes GmbH.
