Description 3-D Life FG-Dextran is a component of the 3-D Life Hydrogel system. It contains maleimide-functionalized dextran (Mal-Dextran) that can be used together with PEG-Link (Cat. No. L50-1 or L50-3) or CD-Link (Cat. No. L60-1 or L60-3) to set up fast gelling, cell-compatible hydrogels. When crosslinked with PEG-Link or CD-Link, Dextran-based 3-D Life Hydrogels can be dissolved by the addition of dextranase (3-D Life Dextranase, Cat. No. D10-1), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation. NOTE: Dextran hydrogels crosslinked with HyLink cannot be dissolved by dextranase. The included 10x CB Buffer (pH 5.5) is a buffer to control pH and osmotic conditions during gel formation in the presence of cells. For instructions, please consult the General Protocol GP-1 "Preparation of 3-D Life Fast Gelling Hydrogels" and the 3-D Life Hydrogels User Guide, which may be downloaded below. Application 3D cell cultures; embedment and immobilization of cells or tissues and subsequent recovery of specimens by hydrogel dissolution. Quantity Allows formation of up to 6 ml 3-D Life Hydrogel depending on the stiffness of the gel, if used together with thiol-functionalized polymers (3-D Life PEG-Link or CD-Link). Components Material Quantity Concentration of reactive groups Mal-Dextran, Lyophilized 3 x 170 µl* 30 mmol/L MSDS 10x CB Buffer (pH 5.5) 600 µl n/a MSDS Water 4 x 1500 µl n/a MSDS *Volume after reconstitution of lyophilizate. Resources Product Datasheet (PDS): 3-D Life FG-Dextran Component 3-D Life User Guide & Protocols 3-D Life Biomimetic Hydrogels Page 3-D Life Hydrogels User Applications 3-D Life Hydrogels Product References Application Notes Application Note: Epithelial Cyst Formation in 3-D Life Hydrogels Application Protocol: MDCK Cyst Formation in 3-D Life Hydrogels Ilex Life Sciences LLC is an authorized distributor of Cellendes GmbH.
