Description 3-D Life HyLink is a component of the 3-D Life Hydrogel system. The HyLink molecule consists of hyaluronic acid (hyaluronan) carrying thiol groups along its backbone. When combined with SG-Polymers of the 3-D Life Hydrogel system, thiol groups on HyLink form stable thioether bonds with the thiol-reactive groups on the polymers, which results in the formation of the hydrogel. HyLink can be used with 3-D Life functionalized polymers SG-PVA and SG-Dextran. For using this product please consult General Protocol GP-4 "Preparation of 3-D Life Hyaluronic Acid (HA) Hydrogels" and the 3-D Life Hydrogels User Guide, which may be downloaded below. Application 3-D Life HyLink is used together with thiol-reactive SG-Polymers of the 3-D Life Hydrogel system to establish 3D cell cultures. Quantity Cat. No. L70-1: Sufficient for 1.6 ml hydrogel of a crosslinking strength of 1.2 mmol/l when used with SG-Polymers of the 3-D Life Hydrogel system. Cat. No. L70-3: Sufficient for 5 ml hydrogel of a crosslinking strength of 1.2 mmol/l when used with SG-Polymers of the 3-D Life Hydrogel system. Components Cat. No. L70-1: Material Quantity Concentration of reactive groups HyLink, Lyophilized 200 µl* 10 mmol/L* MSDS Water 600 µl n/a MSDS *Volume after reconstitution of lyophilizate. *Concentration after reconstitution of lyophilizate. Cat. No. L70-3: Material Quantity Concentration of reactive groups HyLink, Lyophilized 3 x 200 µl* 10 mmol/L* MSDS Water 600 µl n/a MSDS *Volume after reconstitution of lyophilizate. *Concentration after reconstitution of lyophilizate. Resources Product Datasheet (PDS): 3-D Life HyLink (200 µl) Product Datasheet (PDS): 3-D Life HyLink (3x 200 µl) 3-D Life User Guide & Protocols 3-D Life Biomimetic Hydrogels Page 3-D Life Hydrogels User Applications 3-D Life Hydrogels Product References Product References Jung, Nathalie et al. “Non-invasive analysis of pancreas organoids in synthetic hydrogels defines material-cell interactions and luminal composition.” Biomaterials science vol. 9,16 (2021): 5415-5426. doi:10.1039/d1bm00597a Georgakopoulos, Nikitas et al. “Long-term expansion, genomic stability and in vivo safety of adult human pancreas organoids.” BMC developmental biology vol. 20,1 4. 26 Feb. 2020, doi:10.1186/s12861-020-0209-5 Ilex Life Sciences LLC is an authorized distributor of Cellendes GmbH.
