Description The 3-D Life ToGro Hydrogel Kit contains reagents for the preparation of 3-D Life hydrogels with a soft stiffness (400 Pa; shear modulus) and a RGD Peptide concentration of 0.4 mmol/L. The gel composition allows three-dimensional spreading and migration of most cells. The gel is formed by the crosslinking of modified dextran carrying the cell adhesion motif RGD (RGD-Dextran) with CD-Link. When the two reagents are combined, thiol groups on CD-Link form stable thioether bonds with thiol-reactive groups on RGD-Dextran resulting in gel formation within 20 min. The formation of hydrogel is performed at physiological pH for optimal cell compatibility. CD-Link is composed of polyethylene glycol and a matrix metalloprotease (MMP)-cleavable peptide. The MMP-cleavable peptide is designed for a broad range of MMP cleavage including MMPs MMP1, MMP3, MMP7 and MMP9. It allows cells to spread and migrate within the gel if they express the indicated MMPs. 3-D Life ToGro hydrogels can be dissolved by the addition of dextranase (3-D Life Dextranase Cat. No. D10-1), which allows recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or further cultivation. For preparation of gels follow the instructions of the 3-D Life ToGro Protocol GP-3 “Preparation of 3-D Life ToGro Hydrogel”. The document can be downloaded below. Application 3D cell culture: 3-dimensional cultivation of various cell types, spheroids, tissue samples and similar requiring a cell-adhesive and cell-degradable matrix for cell growth and differentiation. Quantity Allows formation of 2.4 ml 3-D Life ToGro Hydrogel. Components Material Quantity Concentration of reactive groups RGD-Dextran, lyophilized 2 x 870 µl* n/a MSDS CD-Link, lyophilized 200 µl* 20 mmol/L* MSDS Reconstitution Buffer 2 x 900 µl n/a MSDS Water 600 µl n/a MSDS *Volume/concentration after reconstitution of lyophilizate. Resources Product Datasheet (PDS): 3-D Life ToGro 3-D Life User Guide & Protocols 3-D Life Biomimetic Hydrogels Page 3-D Life Hydrogels User Applications 3-D Life Hydrogels Product References Product References Chen, Jeng-Chang et al. “Enteric neurospheres retain the capacity to assemble neural networks with motile and metamorphic gliocytes and ganglia.” Stem cell research & therapy vol. 14,1 290. 5 Oct. 2023, doi:10.1186/s13287-023-03517-y Ilex Life Sciences LLC is an authorized distributor of Cellendes GmbH.
