SuperSignal MaxiSignal Maximum Sensitivity Substrate is designed to detect antibodies and associated antigens directly or indirectly labeled with horseradish peroxidase (HRP). The principle is that proteins or nucleic acids were transferred to the imprinted membrane after electrophoresis, and the target proteins on the membrane were bound by primary antibody and secondary antibody labeled with HRP, or the nucleic acids on the membrane were bound directly or indirectly by probes labeled with HRP. After washing the membrane, the ECL working solution prepared by the product was used to incubate the membrane at room temperature for several minutes. The imprinted membrane was wrapped with plastic wrap and fixed to the X-ray exposure Cassette. Then the X-ray film is pressed on the membrane in a darkroom and exposed for several seconds to several hours. After development and fixing, protein or nucleic acid bands can be clearly displayed on the X-ray film.This kit has a unique luminescent/enhanced substrate system, While reducing the exposure background, the introduction of a new oxidant greatly improves the stability of the kit, which can achieve low fick-grade western blotting detection. In addition to X-rays, can also be detected by the automatic imaging system. Features High sensitivity and high signal-to-noise ratio, it can detect low fick-grade antigens. Long duration of luminescence, fluorescence can make X-ray film sensitive for more than 8 hours, especially suitable for detecting trace protein or nucleic acid. Higher dilution ratio antibodies can be used to greatly save antibodies. Dilution ratio of primary antibody (liquid storage concentration 1mg/mL) : 1:1000-1:100000. Dilution ratio of secondary antibody (liquid storage concentration 1mg/mL): 1:100000-1:500000. Applications Chemiluminescence ELISA Western Blot Dot Blot-DNA/RNA Southern blot-DNA Northern blot-RNA Specifications Recommended Antibody Concentrations primary antibody (liquid storage concentration 1mg/mL): 1:1000-1:100000 secondary antibody (liquid storage concentration 1mg/mL): 1:100000-1:500000 Signal Duration < 8 h Substrate Properties Chemical Substrate Substrate Type HRP (Horseradish Peroxidase) Substrate Sensitivity low fick-grade antigens Components Components No. Name 36224ES10(10 mL) 36224ES60(100 mL) 36224ES70(200 mL) 36224ES76(500 mL) 36224-A MaxiSignal ECL - Reagent A 5 mL 50 mL 100 mL 250 mL 36224-B MaxiSignal ECL - Reagent B 5 mL 50 mL 100 mL 250 mL Storage The product should be stored at 2℃~8℃ for one year. Notes: 36224-A should be stored away from the light! Figures Effect Contrast of Maximum Sensitivity Substrate Figure 1. Comparison of detection effect between YEASEN Maxisignal substrate and West Femto of the same level substrate of other brands YEASEN ECL reagent has a better use effect than other brands of the same level of products. FAQ Q: Can this product detect antigens at the high femtogram level? A: No. This product has extremely high sensitivity and a high signal-to-noise ratio, and can detect antigens as low as picograms. Q: How long will this product last? A: The general signal can last for more than 8 hours. Q: What is the dilution ratio of the primary antibody used in this product? A: Dilution factor of primary antibody (storage concentration 1mg/ml): 1:5000 - 1:100000. Q: What is the dilution ratio of the secondary antibody used in this product? A: Dilution factor of the secondary antibody (storage concentration 1mg/ml): 1:100,000 - 1:500,000. Documents: Safety Data Sheet 36224_MSDS_HB220420_EN_1652931562506.PDF 36224_MSDS_HB220420_EN_1652931562507.PDF 36224_MSDS_HB220420_EN_1652931562550.PDF 36224_MSDS_HB220420_EN_1652931562816.PDF Manuals 36224_Manual_HB221212_EN.pdf Citations & References: [1] Zhang Y, Liu F, Feng Y, et al. CircRNA circ_0006156 inhibits the metastasis of prostate cancer by blocking the ubiquitination of S100A9 [published online ahead of print, 2022 Jun 27]. Cancer Gene Ther. 2022;10.1038/s41417-022-00492-z. doi:10.1038/s41417-022-00492-z(IF:5.987) [2] Yu Y, Fang L. CircRPAP2 regulates the alternative splicing of PTK2 by binding to SRSF1 in breast cancer. Cell Death Discov. 2022;8(1):152. Published 2022 Apr 2. doi:10.1038/s41420-022-00965-y(IF:5.241) [3] Cao Y, Sun M, Wang J, Hu X, He W, Su J. Phenotypic and genetic characterisation of an emerging reovirus from Pekin ducks in China. Sci Rep. 2019;9(1):7784. Published 2019 May 23. doi:10.1038/s41598-019-44178-3(IF:4.011) [4] Sun L, Song F, Liu H, et al. The novel mutation P36R in LRP5L contributes to congenital membranous cataract via inhibition of laminin γ1 and c-MAF. Graefes Arch Clin Exp Ophthalmol. 2020;258(12):2737-2751. doi:10.1007/s00417-020-04846-x(IF:2.396)
