This Inorganic Pyrophosphatase (PPase) Residue Detection Kit uses the double-antibody sandwich ELISA method to detect residual PPase in mRNA vaccine products. PPase catalyzes the conversion of pyrophosphate to phosphate, promoting higher mRNA yields in production. However, PPase must be removed during purification to ensure product quality, and its residues must be tested to confirm effective removal. The detection process involves adding both PPase standard and test samples to Anti-PPase-coated microtiter strips. Afterward, a biotin-labeled anti-PPase antibody and Streptavidin-HRP are added to form an antibody-antigen complex. TMB substrate is then added, and the color change from blue to yellow indicates the PPase amount in the sample. The color intensity is directly proportional to the PPase concentration. The kit’s detection range is 0.25–16 ng/mL, with a lower detection limit of 0.165 ng/mL. Feature Highly sensitive: Detecting as little as 0.165 ng/mL of Inorganic pyrophosphatase residuals in in-process and final biological samples. Ensures accuracy: The Inorganic pyrophosphatase residuals can be accurately detected with a recovery of 75%-125%. Specificity: Cooperate use with YEASEN Inorganic pyrophosphatase and can accurately detect the residuals. Stable: The difference between lot-to-lot is low, the kit property is not impacted under 37℃ for 7 days. Easy signal collection: Using Biotin-Streptavidin system, the signal can be stably enlarged. Application Residual Inorganic pyrophosphatase test in biological products; Residual Protein Detection in mRNA Vaccine and Therapeutic Production; Purification Validation in Nucleic Acid Synthesis and In Vitro Transcription (IVT) Specification Cat.No. Size Price 36706ES48 48 T $764 36706ES96 96 T $1376 Property Detection range 0.25~16 ng/mL Sensitivity 0.165 ng/mL Assay Time 3.5 h Assay Principle Double antibody sandwich method Dilution linearity Difference with undiluted sample
