Hieff Trans™ Liposomal Transfection Reagent (Ver.2000) _ 40802ES
Hieff Trans™ Liposomal Transfection Reagent is a versatile liposome transfection reagent, suitable for DNA, RNA, and oligonucleotide transfection, with high transfection efficiency for most eukaryotic cells. Its unique formula allows it to be added directly to the medium, and the presence of serum does not affect transfection efficiency, which reduces the damage to cells caused by serum removal. There is no need to remove the nucleic acid-Hieff Trans™ complex or replace it with a fresh medium after transfection, and it can also be removed after 4-6 hours. Hieff Trans™ is supplied in sterile liquid form. Usually, for 24-well plate transfection, about 1.5 μL each time, 1 mL of Hieff Trans™ can do about 660 transfections; for a 6-well plate, about 6 μL each time, 1 mL of Hieff Trans™ can do about 660 transfections. 160 transfections. Feature High Efficiency: Enables transfection in most eukaryotic cells, achieving over 90% efficiency in common cell lines. Low Toxicity: Transfected cells maintain healthy morphology while expressing abundant target protein. Easy to Use: Lipid complexes can be directly added to serum-containing culture media. Versatile Applications: Suitable for both transient and stable transfection experiments. Application Cell Transfection Specification Form Liquid Serum Compatible Yes Cell Type Established Cell Lines Sample Type Plasmid DNA, Synthetic siRNA Transfection Technique Lipid-Based Transfection Components Components No. Name 40802ES01 40802ES02 40802ES03 40802ES08 40802 Hieff Trans™ Liposomal Transfection Reagent 100 μL 0.5 mL 1 mL 5×1 mL Shipping and Storage The product is shipped with ice packs and can be stored at 2-8ºC for one year. Do not freeze! Figures 1. High transfection efficiency Figure 1. Efficient DNA transfection in 293 cells. Transient transfection was performed in 12-well plates using a total of 1 μg plasmid (0.5 μg GFP + 0.5 μg RFP) with 3 μL of transfection reagent. Cited from: Communications Biology volume 5, Article number: 1296 (2022) 2. High transfection siRNA Transfection Figure 2. Efficient AMPK gene silencing in HK2 cells. HK2 cells were transfected in 6-well plates with 100 pmol siRNA targeting AMPK using 6 μL of transfection reagent. High silencing efficiency (+++) was observed with no detectable cytotoxicity Each reagent was used to transfect the target cell line（Hela cell line in Figure 1a, DF-1 cell line in Figure 1b）in a 96-well format. GFP expression was analyzed 48 hours posttransfection. Hieff Trans™ Liposome Transfection Reagent provided higher GFP transfection efficiency than the competitive brand products. 3. Figure 3. Comparison of transfection efficiency between Hieff Trans™ Liposome Reagent and other brands. (A).Hieff Trans™ and supplier P* were used to transfect HeLa and DF-1 cells in 96-well plates. GFP expression was measured 48 hours post-transfection. Hieff Trans™ consistently achieved higher GFP transfection efficiency than the other products. 4. Validated Cell Lines 293T 293FT HEK293 HEK 293T Caco2 COS-7 DF-1 Lenti X-293T HeLa HepG2 LM3 MCF-7 MDA-MB-231 MEF NCI-H1975 NIH-3T3 PC12 Raw264.7 Vero H9c2 Hepa1-6 MCF10A CHO 5-8F BV-2 A549 3t3 H520 HUVEC C6 HaCaT N2A SGC-7901 H9 MKN-28 Hep 3B SMCC7721 RKO C2C12 More.. Figure 4. List of cell lines successfully transfected with Hieff Trans™ Liposomal Transfection Reagent (Under continuous update). 5.Case FAQs: Q1: Can serum be present when preparing nucleic acid transfection reagent complex? A: The presence of serum will affect the formation of liposomes. It is recommended to use serum-free medium (usually MEM medium) when preparing nucleic acid transfection reagent complexes. Q2: Can the transfection reagent be frozen? A: No. This reagent must be stored at 2-8 ℃, and care should be taken to avoid repeatedly opening the cap for a long time, as long-term opening of the cap will cause liposome oxidation and affect the transfection efficiency. Q3: What should I pay attention to when using Hieff Trans™ Liposome Nucleic Acid Transfection Reagent? A: 1) During the transfection operation, it is better that the cell confluence reaches 80%-95%, and the specific plating density is determined according to the situation of the cells; 2) Using high-purity DNA helps to obtain higher transfection efficiency; 3) DNA and transfection reagents are required to be diluted with serum-free medium when preparing transfection complexes; 4) Antibiotics cannot be added to the medium during transfection; 5) The DNA concentration and the amount of cationic liposome reagent should be optimized for the first use to obtain the maximum transfection efficiency. The ratio of DNA to transfection reagent is generally recommended to be 1:2-1:3. Q4: Does it need to be terminated after transfection? A: No need. Liposome complexes are stable for 6 hours. If the cell medium is not changed before transfection, in order to ensure the nutrients required for normal cell growth, it is necessary to change to a new medium after 4 to 6 hours. However, if the medium has been changed before transfection, it is not necessary to change the medium after liposome transfection. Q5: What should I pay attention to if I want to improve the transfection efficiency? A: a: The density of cells at the time of transfection is 90%-95%. b: During transfection, use MEM serum-free medium for nucleic acid and liposome dilutions. c: The medium can be changed 4-6h after transfection. Q6: Can co-transfection of DNA and siRNA be performed? How's the effect? A: Co-transfection can be performed, but it is recommended to perform separate transfection, and DNA transfection should be performed 6 hours after siRNA. If operated together, the siRNA transfection efficiency will be worse. Q7: Can the transfection reagent be used for lentiviral packaging transfection? A: Lentiviral packaging is possible, but the efficiency of lentiviral packaging is not necessarily related to the efficiency of transfection, but also related to the selection of packaging plasmids and the ratio between plasmids. Q8: Can Hieff Trans™ Liposome Nucleic Acid Transfection Reagent be used for transfection of suspension cells? A: Hieff Trans™ Liposome Nucleic Acid Transfection Reagent can be used for suspension cell transfection, see Protocol for details. In addition, we have also launched a transfection reagent specifically for suspension cells (Cat No. 40805, Liposome nucleic acid transfection reagent for suspension cells). 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