Coelenterazine 400a is a derivative of native coelenterazine and serves as an excellent substrate for Renilla luciferase (Rluc). However, it cannot be oxidized by Gaussia luciferase (Gluc). Its maximum emission wavelength is around 400 nm, which minimizes signal interference with the GFP receptor protein. This makes it an important coelenterazine-based substrate for BRET (bioluminescence resonance energy transfer) studies. Features No radiation, almost harmless to living organisms. Bioluminescence, no excitation light source. So sensitive, you can detect it in a few hundred cells. Good penetration, 3-4cm tissue depth can still be detected. High signal-to-noise ratio, strong fluorescence signal and good anti-interference. Applications In the tumorigenesis experiment in nude mice, the tumor growth was observed without invasion in real time, without tumor stripping measurement. To test the effect of the administration on tumor growth or metastasis. The localization and distribution of foreign cells in animals were detected. The target gene or promoter of the target gene is fused to the luciferase gene to detect changes in gene expression during drug treatment or disease progression. Monitoring stem cell transplantation, survival and proliferation; Trace the distribution and migration of stem cells in vivo. Specifications English synonym Coelenterazine 400a CAS NO. 70217-82-2 Formula C26H21N3O Molecular weight 391.48 g/mol Appearance Yellow to orange powder Solubility Soluble in methanol and ethanol, insoluble in DMSO Purity ≥ 98% Components Components No. Name 40905ES02 40905ES03 40905 Coelenterazine 400a 1×500 μg 2×500 μg Shipping and Storage -20℃ keep away from light and dry storage, preferably stored in an inert gas environment, avoid contact with air; long-term storage at -70℃. Valid for 1 years. Figures Documents: 40905_MSDS_HB241231 40905_Manuals_Ver.EN20241231 Citations & References:
