MDCK Host Cell DNA Residue Detection Kit is used for the quantitative analysis of MDCK host cell DNA residuce in intermediate samples, semi-finished and finished products of vaious biological products. This kit adopts Taqman fluorescent probe and the polymerase chain reaction (PCR) method, which has fg level minimum detection limit and can specifically and quickly detect the residual MDCK cell DNA. The kit needs to be used together with the the Residual DNA Sample Preparation Kit (Cat# 18461ES). Feature Compliance with regulations: the products are fully validated according to the requirements of Chp, USP, ICH, etc., and their performance is in line with Chinese and foreign regulations and standards; Guarantee of quality: the raw materials of the kits are all self-developed, and the qPCR Mix and other enzyme products are produced in an ultra-clean enzyme factory; High sensitivity: the limit of quantification can reach 0.5 fg/μL level; High precision: high intra-batch repeatability and low inter-batch variation; Strong exclusivity: specific detection of residual DNA in MDCK cells without interference from other exogenous genomic DNA; Strong anti-interference: add internal control (IC), easy to exclude sample interference, reaction preparation abnormalities and other factors. Application Residual MDCK Host Cell DNA test in biological products. Specification Sensitivity 0.5 fg/μL (range 3fg/μL~300pg/μL) Assay Time ~1.5 hours Assay Principle Fluorescent probe qPCR method Required Instruments Real-time fluorescence quantitative PCR system Components Components No. Name 41319ES50-EN 41319ES60-EN 41319-A MDCK qPCR Mix 0.75 mL 1.5 mL 41319-B MDCK Primer&Probe Mix 200 μL 400 μL 41319-C DNA Dilution Buffer 1.8 mL×2 1.8 mL×4 41319-D MDCK DNA Control（30 ng/μL） 25 μL 50 μL 41319-E IC* 50 μL 100 μL *IC：Internal control. Storage This product should be stored at -25~-15℃ for 2 years. Both 41319-A and 41319-B should be stored protected from light. Figures Limit of quantification MDCK DNA was detected at 3 fg/uL, 1 fg/uL, 0.5 fg/uL, and 0.1 fg/uL with 10 replicates for each concentration. The results showed that at concentrations of 0.5 fg/uL and above, the CV was
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