Sf9 and Baculovirus DNA Residue Detection Kit is used for quantitatively analysing and detecting Sf9 host cell DNA and baculovirus DNA residues in the development and production of a variety of biologics, such as gene therapies, recombinant proteins and vaccines, using the baculovirus-insect cell (Sf9) expression system. This kit is based on the principle of fluorescence quantitative PCR by probe method, and uses multiplex qPCR method to detect Sf9 and baculovirus residual DNA, respectively. The limit of quantification can be up to 1 fg/μL for Sf9, and the limit of quantification for baculovirus can be up to 7 copies/uL.The kit needs to be used together with the the Residual DNA Sample Preparation Kit (Cat# 18461ES). Validation Report Feature Compliance with regulations: the products are fully validated according to the requirements of Chp, USP, ICH, etc., and their performance is in line with Chinese and foreign regulations and standards; Guarantee of quality: the raw materials of the kits are all self-developed, and the qPCR Mix and other enzyme products are produced in an ultra-clean enzyme factory;High sensitivity: the lower limit of quantification (LLOQ) was 1fg/μL for Sf9 and 7copies/uL for AcNPV;High precision: high intra-batch reproducibility CV
