The Cell Species Identification Kit (Multiplex PCR Method) is designed for the identification of cell substrate origins, ensuring correct cell line identity and absence of cross-contamination from other cell lines. It enables quality control of raw materials in cell-based biopharmaceuticals. This kit is based on multiplex PCR amplification of mitochondrial genes from animal cells, including cytochrome b, cytochrome c oxidase I (COX I), and cytochrome c oxidase II (COX II). Species origin is determined by analyzing the amplification products via agarose gel electrophoresis. Features Broad Species Compatibility – Accurately detects 9 different cell species, including human (HEK293), mouse (SP2/0), bovine (MDBK), porcine (PK15), canine (MDCK), Chinese hamster (CHO), African green monkey (Vero), feline (CRFK), rat (C6) and rhesus monkey (LLC-MK2). High Specificity – Reliably identifies contaminating cells even in the presence of primary cells (HEK293, MDCK, Vero, CHO). Reliable Results – Based on amplicon band number and size for clear interpretation. High Quality & Stable Supply – Ensures consistent performance and reliable availability. Specifications Cat NO. 41341ES25 / 41341ES50 Size 25 T / 50 T Components category Components No. Name 41341ES25 41341ES50 Part I 41341-C Cell Extraction Buffer 2 × 1.25 mL 4 × 1.25 mL Part II 41341-A 2× Multiplex PCR Buffer 312.5 μL 625 μL 41341-B Primers Mix 112.5 μL 225 μL 41341-D Proteinase K (20 mg/mL) 50 μL 100 μL 41341-E DL500 DNA Marker 125 μL 250 μL 41341-F DEPC-Treated Water 1.25 mL 2 × 1.25 mL 41341-G Positive Control 25 μL 50 μL Application Cell species identification Figures 1. Broad Species Compatibility Figure 1. Broad species compatibility. Clear amplification results were obtained across different species, including human (HEK293), mouse (SP2/0), bovine (MDBK), porcine (PK15), canine (MDCK), Chinese hamster (CHO), African green monkey (Vero), feline (CRFK), rat (C6), and rhesus monkey (LLC-MK2).
Related
Related
Related
Related