In vitro cell culture often involves the addition of antibiotics to the culture medium to prevent microbial contamination. Among these, penicillin is the most widely used. Penicillin, streptomycin, and neomycin have a good combined antibacterial effect against both Gram-positive and Gram-negative bacteria, which can prevent bacterial contamination in cell culture. This product has been sterilized by filtration through a 0.1 μm filter and contains Penicillin G sodium salt at 5 kU/mL, streptomycin sulfate at 5 mg/mL, and neomycin sulfate at 10 mg/mL. It is provided as a 100X concentrated solution, which requires dilution before use, and it should be used without repeated freeze-thaw cycles. Yeasen company provides three commonly used multi-antibiotic solutions for cell culture: 60162ES, 60290ES and 60291ES. The differences among these three products are as follows: Product Number 60162ES 60290ES 60291ES Product Name Penicillin-Streptomycin Solution, 100×, Suitable for Cell Culture Penicillin-Streptomycin-Neomycin Solution(PSN), 100×, Suitable for Cell Culture Penicillin-Streptomycin-Amphotericin B Solution, 100×, Suitable for Cell Culture Main Active Ingredients Penicillin 10,000 U/mL; Streptomycin 10,000 µg/mL Penicillin G Sodium Salt 5 kU/mL; Sulfate Streptomycin 5 mg/mL; Sulfate Neomycin 10 mg/mL Penicillin 10,000 IU/mL; Sulfate Streptomycin 10 mg/mL; Amphotericin B 25 µg/mL Solvent Sterile Water PBS Buffer Solution Cell Culture Medium Water Recommended Usage Concentration Dilute to 1× Applicable Scenarios Effectively inhibits the growth of most Gram-positive and Gram-negative bacteria Have a good combined antibacterial effect against Gram-positive and Gram-negative bacteria Penicillin and streptomycin inhibit bacterial protein synthesis, and amphotericin B inhibits the growth of fungi For other antibiotics, please refer to the related topic of Antibiotic User Manual. Features Standardized production Penicillin G Sodium Salt 5 kU/mL; Sulfate Streptomycin 5 mg/mL; Sulfate Neomycin 10 mg/mL Applications Cell culture A good combined antibacterial effect against Gram-positive and Gram-negative bacteria Specifications Size 100 mL Appearance Liquid pH 6.1-6.4 Components Components No. Name 60290ES60 60290 Penicillin-Streptomycin-Neomycin Solution(PSN), 100×, Suitable for Cell Culture 100 mL Shipping and Storage The Penicillin-Streptomycin-Neomycin Solution products be stored at -25~-15℃ for 3 years, protected from light. Documents: Safety Data Sheet 60290_MSDS_HB250210_EN.pdf Manuals 60290_Manual_HB250210_EN.pdf