BlotⅠis a part of mini electrophoresis system, which also contains Mini Vertical Transfer Slot that running SDS-PAGEgel and Native-PAGE gel .BlotⅠaccommodates 2 blot clips, uses for blotting protein and RNA sample in the polyacrylamide and agarose gel. Cooling unit belongs to the standard accessories of BlotⅠ, which after being freezed to absorb the heat generated during the operation of blotting. The built-in cooling unit avoids the trouble of connection and the cost of installing the external cooling-recycling system. Other features include easy-to-use lock mechanism installed in gel clamp, gel clamp with color marker, direction positioning, special-designed plug-in-out mechanism. All these features make the BlotⅠa perfect electrophoresis system that is easy to use. Features Compact Vertical DesignP：Space-saving structure, ideal for small-scale Western Blot experiments with easy operation. High-Efficiency Transfer：Optimized electric field ensures uniform and efficient protein transfer (>90% efficiency). Multiple Transfer Modes：Compatible with wet and semi-dry transfer methods, supporting PVDF/NC membranes and various buffer systems. Modular Assembly：Quick gel-membrane sandwich setup; transparent cover allows real-time monitoring with leak-proof safety. Durable Construction：High-strength, corrosion-resistant plastic with oxidation-resistant electrodes for long-term reliability. Broad Applications：Suitable for protein immunoblotting, antibody detection, and teaching labs, meeting diverse research needs. Applications Western Blot Protein Transfer Teaching & Training Protein-Protein Interaction Research Post-translational Modification Studies Specifications Electrophoresis core Polycarbonate Gel sandwich clamp Polycarbonate Electrophoresis electrode Platinum wire (made from platinum ingot) Buffer tank and upper lid Polycarbonate Cooling unit Polyethylene and cold storage agent Cell size 18 cm (L) x 13 cm (W) x 16 cm (H) Gel clamp size 10 cm x 11 cm Maximum gel size 7.5 cm x 10 cm Buffer volume (with cooling unit) 850 ml Buffer volume (without cooling unit) 650 ml Components Components No. Name 80220ES05 80220 BlotⅠMini Vertical Transfer Slot 1 Unit Figures Operation instructions Put cooling unit into the refrigerator with temperature -20°C, and put it back after usage. 1.Prepare the buffer. (Please refer to formula mentioned in section 3.3, it is easy to diffuse the heat when the buffer is freeze to 4°C) 2.Cut the filter paper and membrane according to the size of gel, please wear the glove to avoid pollution. Equilibrate the gel and immerse the membrane and filter paper as well as the macroporous sponge (From 15 minutes to 1 hour, depended on the thickness of the gel). 3.Prepare the sandwich clamp and put on the clean desk with the black side downward. Put a ready-wet macroporous sponge on the black part of clamp. Put the wet filter paper on the macroporous spong. Put the equilibrated gel on the filter pater (Remove the air bubble between the gel and filter paper). Put the immersed membrane on the gel (Remove the air bubble between the membrane and gel). Put the filter paper on the membrane and remove the air bubble, add the transfer lining. Note: In order to achieve the best blot result, it is key to remove the air bubble by glass bar stirring. 4. Tighten the clamp and do not move the gel and filter paper sandwich, then use the white sliding latch to lock the clamp. 5. Insert the clamp into the blot electrophoresis core, and repeat the process to make another sandwich gel. 6. Put the cold cooling unit in the tank and fill up the tank by buffer. 7. Put the stirring bar into the buffer to maintain temperature and equilibration of the ion strength, set the most suitable speed to distribute the ion evenly. 8. Close the safety lid and plug the power supply cable into power supply and start the blot. 9. Disassemble the sandwich when the electrophoresis ends, take out the membrane and continue the next step. Use the neutral detergent to rinse the electrophoresis tank, blot clip, macroporous sponge, and then again use the ion-removing water to rinse. If the transfer is being done under acidic conditions, switch the position of the gel to the membrane and place the membrane on the cathode of the gel.Under acidic conditions, the protein migrates to the opposite direction, the direction of negative electrode, do not reverse the electrode otherwise it may be damaged.
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