Product Name Cat# Size Recombinant Human IL-3 Protein 90104ES10 10 μg 90104ES50 50 μg 90104ES60 100 μg 90104ES76 500 μg (5×100 μg) 90104ES80 1 mg (10×100 μg) Background IL-3, also known as Interleukin-3, is a cytokine that is produced by activated T cells and mast cells. It plays a crucial role in regulating the growth, differentiation, and function of various cell types in the immune system. human IL-3 is an important cytokine that regulates the growth, differentiation, and function of immune cells. Its study contributes to our understanding of immune system regulation and its potential applications in immunotherapy and disease treatment. Product Properties Synonyms Interleukin-3, IL-3, Hematopoietic growth factor, MCGF, Multipotential colony-stimulating factor, P-cell-stimulating factor Accession P08700 GeneID 3562 Source E.coli-derived human IL-1alpha protein, Ala20-Phe152. Molecular Weight Approximately 15.0 kDa. AA Sequence APMTQTTSLK TSWVNCSNMI DEIITHLKQP PLPLLDFNNL NGEDQDILME NNLRRPNLEA FNRAVKSLQN ASAIESILKN LLPCLPLATA APTRHPIHIK DGDWNEFRRK LTFYLKTLEN AQAQQTTLSL AIF Tag None Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% by SDS-PAGE and HPLC analyses. Biological Activity The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.1 ng/mL, corresponding to a specific activity of > 1.0 × 107 IU/mg. Fully biologically active when compared to standard. Endotoxin < 0.01 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. Shipping and Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. 2-7 days, 2 ~8 °C under sterile conditions after reconstitution. 3-6 months, -85~-65℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Cautions 1.For your safety and health, please wear lab coats and disposable gloves for operation. 2.For research use only. Assay Data SDS- PAGE The purity of Human IL-3 is greater than 95% as determined by SDS-PAGE. SEC-HPLC The purity of Human IL-3 is greater than 95% as determined by SEC-HPLC. Cell Based Assay The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.1 ng/mL, corresponding to a specific activity of > 1.0 × 107 IU/mg. Documents: Manuals 90104_Manual_Ver.EN20250708.pdf
Related
Related
Related
Related