Interleukin-6 (IL-6) is a pleiotropic cytokine that plays a critical role in host defense by modulating immune and inflammatory responses. Produced by T cells, monocytes, fibroblasts, endothelial cells, and keratinocytes, IL-6 exerts diverse biological functions. It stimulates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces the expression of hepatic acute-phase proteins, and regulates bone metabolism. Signaling is transduced through the IL-6 receptor system, which comprises two chains: IL-6Rα and gp130. This product is recombinant human IL-6 supplied as a lyophilized powder with high activity, high purity, and low endotoxin. Product Information Product Name Catalog No. Size Recombinant Human IL-6 Protein 90107ES08 5 μg 90107ES10 10 μg 90107ES20 20 μg 90107ES50 50 μg 90107ES60 100 μg 90107ES80 1 mg Specifications Synonyms Interleukin-6, BSF2, HSF, IFNB2, IL6 Source Species Human Expression Sequence Val30-Met212 Accession P05231 Tag No Tag Expression System E. coli Molecular Weight Approximately 20.8 kDa. Purity > 95% as determined by SDS-PAGE Endotoxin < 0.01 EU per μg by the LAL method. Formulation Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4. Appearance Lyophilized Powder Biological Activity The ED₅₀ as determined by a cell proliferation assay using human TF-1 cells is 0.1–0.8 ng/mL. Reconstitution Centrifuge the vial before opening to bring contents to the bottom. Reconstitute with sterile distilled water or PBS to a concentration of 0.1–1.0 mg/mL, and dilute further as needed for subsequent experiments. For long-term storage, a carrier protein (0.1% BSA) is recommended in the dilution buffer. Aliquot into single-use volumes and store at –80°C; avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Application SDS-PAGE Figure 1. Human IL-6 Protein on SDS-PAGE under reduced (R) & Non-reducing (NR) condition. The purity is greater than 95%. Bioactivity Figure 2. The ED₅₀ as determined by a cell proliferation assay using human TF-1 cells is 0.1–0.8 ng/mL. Storage Store at –25 to –15°C. Valid for 1 year from the date of receipt. After reconstitution, store under sterile conditions at 2–8°C for up to 7 days, or at –85 to –65°C for up to 3 months. Documents: Safety Data Sheet 90107_MSDS_HB260811 Manuals: 90107_Manual_HB20260811
