Product Name Cat# Size Recombinant Human IL-10 Protein 90109ES05 2 μg 90109ES10 10 μg 90109ES50 50 μg 90109ES60 100 μg 90109ES76 500 μg Interleukin 10 (IL-10) is an immunosuppressive cytokine produced by a variety of mammalian cell types, including macrophages, monocytes, T cells, B cells, and keratin-forming cells.IL-10 inhibits the expression of pro-inflammatory cytokines such as IL-1 and TNF-alpha. Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune responses. This recombinant human IL-10 is supplied as a lyophilized powder with high activity, high purity, low endotoxin, and no redundant labeling. Specification Synonyms Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, IL10A, TGIF, CSIF；IL10 Source E.coli-derived human IL-10 protein, Ser19-Asn178. Accession P22301 Molecular Weight Approximately 18.6 kDa. AA Sequence SPGQGTQSEN SCTHFPGNLP NMLRDLRDAF SRVKTFFQMK DQLDNLLLKE SLLEDFKGYL GCQALSEMIQ FYLEEVMPQA ENQDPDIKAH VNSLGENLKT LRLRLRRCHR FLPCENKSKA VEQVKNAFNK LQEKGIYKAM SEFDIFINYI EAYMTMKIRN Endotoxin < 1.0 EU/μg by the LAL method. Purity > 95%, SDS-PAGE and HPLC detection. Biological Activity The ED50 as determined by a cell proliferation assay using murine MC/9-2 cells is less than 1 ng/mL, corresponding to a specific activity of > 1.0 × 106 IU/mg. Fully biologically active when compared to standard. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. Physical Appearance White lyophilized powder Storage -25 ~ -15 ° C storage, 1 year expiration date after receipt. After re-solution, store at 2 ~8 °C, 7 days expiration date. After re-dissolving, store at -85~-65°C, 3 months expiration date. Compounding Methods Centrifuge before opening the cap to bring the contents to the bottom. Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL, which can be diluted even further according to subsequent experiments; for long-term storage, it is recommended that carrier protein (0.1% BSA) be added to the dilution buffer; dispense for a single experimental dose and freeze at -80°C to avoid repeated freezing and thawing. Cautions 1. Please operate with lab coats and disposable gloves,for your safety. 2. This product is for research use only. Documents Safety Data Sheet 90109_MSDS_HB251010_EN.PDF Manuals 90109_Manual_Ver.EN20251010.pdf
