IL36 alpha, previously called IL1F6 and FIL1 epsilon (family of IL1 member epsilon), is a member of the IL1 family which includes IL1 beta, IL1 alpha, IL1ra, IL18, and novel family members IL36 Ra (IL1F5), IL36 beta (IL1F8), IL36 gamma (IL1F9), IL37 (IL1F7) and IL1F10. All family members show a 12 beta strand, beta trefoil configuration, and are believed to have arisen from a common ancestral gene. IL36 alpha is an 18 kDa, 160 amino acid (aa) intracellular and secreted protein that contains no signal sequence, no prosegment and no potential Nlinked glycosylation sites. It can be externalized nonspecifically in response to LPS and ATPinduced activation of the P2X7 receptor. Fulllength recombinant IL36 alpha is less active than endogenous IL36 alpha, but trimming of the Ntermini enhances its activity. Mouse IL36 alpha shares 83% aa sequence identity with rat IL36 alpha, 5460% with human, rabbit, equine and bovine IL36 alpha, and 2757% aa sequence identity with other novel IL1 family members. IL36 alpha is mainly found in skin and lymphoid tissues, but also in fetal brain, trachea, stomach and intestine. It is expressed by monocytes, B and T cells. The receptor for IL36 alpha is a combination of IL1 Rrp2 (also called IL1 RL2 or IL1 R6), mainly found in epithelia and keratinocytes, and the widely expressed IL1 RAcP. IL36 alpha, beta and gamma all activate NF kappa B and MAPK pathways in an IL1 Rrp2 dependent manner, and induce production of inflammatory cytokines and chemokines such as CXCL8/IL8. IL36 alpha and other family members are overexpressed in psoriatic skin lesions, and transgenic overexpression of IL36 alpha in skin keratinocytes produces epidermal hyperplasia. IL36 alpha is present in kidney tubule epithelia; it is highly overexpressed in tubulointerstitial lesions in mouse models of chronic glomerulonephritis, lupus nephritis and diabetic nephritis. IL36 alpha is induced by inflammation in adipose tissueresident alternately activated (M2) macrophages, and reduces adipocyte differentiation. Product Properties Synonyms FIL1 epsilon, IL-1 epsilon, IL-1F6, IL-1H1 Accession Q9JLA2 GeneID 54448 Source E.coli-derived Mouse IL-36α,160aa, Met1-His160, with an N-terminal Met. Molecular Weight Approximately 18.0 kDa. AA Sequence MNKEKELRAA SPSLRHVQDL SSRVWILQNN ILTAVPRKEQ TVPVTITLLP CQYLDTLETN RGDPTYMGVQ RPMSCLFCTK DGEQPVLQLG EGNIMEMYNK KEPVKASLFY HKKSGTTSTF ESAAFPGWFI AVCSKGSCPL ILTQELGEIF ITDFEMIVVH Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% by SDS-PAGE and HPLC analyses. Biological Activity The specific activity determined by its ability in a functional ELISA. Immobilized rMuIL-36α at 1 µg/mL can bind recombinant murine IL-1 Rrp2 with a range of 0.15-5 µg/mL. Fully biologically active when compared to standard. Endotoxin < 1.0 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, 5 % trehalose. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. Shipping and Storage The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Cautions 1. Avoid repeated freeze-thaw cycles. 2. For your safety and health, please wear lab coats and disposable gloves for operation. 3. For research use only!
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