Interleukin-9 (IL-9), also known as P40 and MEA (mast cell growth-enhancing activity), is a 30-40 kDa glycosylated member of a cytokine family that includes Interleukins-2, -4, -7, -15, and -21. These proteins utilize heteromeric receptors containing the Common gamma chain ( gamma c) in addition to ligand-specific subunits. IL-9 interacts selectively with IL-9 R which then associates with gamma c to form the functional receptor complex. IL9 contributes to allergic inflammation, autoimmunity-induced inflammation, parasite clearance from the GI tract, and Treg-mediated immune suppression. It enhances the expansion and recruitment of mast cells and eosinophils as well as the production of IgE and Th2 cytokines. It is required for anaphylactic responses to ingested allergens but not to systemic allergens. IL-9 plays multiple roles in the development and function of subsets within the CD4+ T cell lineage. It is expressed by activated Th9, Th17, Treg, and Th2 cells. IL-9 acts as an autocrine growth and activation factor for Th17, Treg, and mast cells. It also can inhibit immune responses by enhancing the suppressive properties of Treg and by recruiting immune-suppressive mast cells to sites of inflammation. Mature rat IL-9 shares 58% and 74% amino acid sequence identity with human and mouse IL-9, respectively. Product Properties Synonyms Cytokine P40; HP40 Accession D4A8l9 GeneID 116558 Source E.coli-derived Rat IL-9, Gln19-Ala144, with an N-terminal Met. Molecular Weight Approximately 14.3 kDa. AA Sequence MQRCSTSWGI QHTSYLIENL KDDPSSKCSC SANVTSCLCL PIPSDDCTTP CFQEGMSQVT NATQQSKFSP FFFRVKRIVE TLKSNKCQFF SCEKPCNQTT AGNTVSFLKS LLKTFQKTEV QVQRSRA Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 97% by SDS-PAGE and HPLC analyses. Biological Activity The ED50 as determined by a cell proliferation assay using murine TS1 cells is less than 10 ng/mL, corresponding to a specific activity of > 1.0 × 105 IU/mg. Fully biologically active when compared to standard. Endotoxin < 0.1 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. Shipping and Storage The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Cautions 1. Avoid repeated freeze-thaw cycles. 2. For your safety and health, please wear lab coats and disposable gloves for operation. 3. For research use only!
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