Interleukin-12 (IL-12), also known as NKSF, TCMF, CLMF and TSF, is a heterodimeric cytokine composed of p35 and p40 subunits. It is produced by monocytes, macrophages, B cells and dendritic cells in response to bacterial lipopolysaccharides and intracellular pathogens. IL-12 signals throughtheIL-12 receptor complex, which is comprised of IL-12 Rβ1 and IL-12 Rβ2. IL-12 induces the proliferation and activation of hematopoietic stem cells, natural killer cells and T- cells. It is indispensible during the development of Th1 cells, leading to the production of IFN-gamma and IL-2. Product Properties Synonyms IL-12a ; IL-12b ; IL-12p35 ; IL-12p40 ; IL12p40 ; IL12a ; p35 ; p40 ;IL12 Accession p35: P43431 p40: P43432 Source CHO-derived Mouse IL-12 protein, IL-12 p35 (Arg23-Ser215) & IL-12 p40 (Met23-Ser335). Molecular Weight 27~29 kDa (p35), 45~50 kDa (p40), on SDS-PAGE under reducing conditions. Tag None Purity > 95% by SDS-PAGE. Biological Activity ED50 < 0.15 ng/ml, measured in a cell proliferation assay using 2D6 cells. Endotoxin < 0.2 EU per 1μg of the protein by the LAL method. Formulation Lyophilized after extensive dialysis against PBS. Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH2O or PBS up to 100 μg/ml. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Shipping and Storage The products are shipped with ice pack and can be stored at -80℃ for 1 year. 1 week, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 °C under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Cautions 1.Avoid repeated freeze-thaw cycles. 2.For your safety and health, please wear lab coats and disposable gloves for operation. 3.For research use only.
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