Product Name Cat# Size Recombinant Human IL-2 Protein 90213ES10 10 μg 90213ES50 50 μg 90213ES60 100 μg 90213ES80 1 mg Interleukin 2 (IL-2) is a potent immunomodulatory lymphokine produced by T cells in response to antigenic or mitogenic stimuli.IL-2/IL-2R signaling is required for T cell proliferation and other essential functions necessary for immune response.IL-2 stimulates B cell, NK cell, lymphokine-activated killer cell, monocyte, macrophage and oligodendrocyte growth and differentiation. This product, Recombinant Human IL-2, is supplied as a lyophilized powder with high activity, high purity and low endotoxin. Specification Synonyms IL2, T-cell Growth Factor, TCGF, Aldesleukin Source E.coli-derived human IL-2 protein, Ala21-Thr153, Tag Not Accession P60568 Molecular Weight Approximately 15.4 kDa. AA Sequence APTSSSTKKT QLQLEHLLLD LQMILNGINN YKNPKLTRML TFKFYMPKKA TELKHLQCLE EELKPLEEVL NLAQSKNFHL RPRDLISNIN VIVLELKGSE TTFMCEYADE TATIVEFLNR WITFCQSIIS TLT Endotoxin < 1.0 EU/μg by the LAL method. Purity > 97% by SDS-PAGE and HPLC. Biological Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 107 IU/mg. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 3.5. Physical Appearance White lyophilized powder Storage -25 ~ -15 ° C storage, valid for 1 year after receipt. After re-solution, store at 2 ~8 °C, 7 days expiration date. After re-dissolution, store at -85~-65°C, 3 months expiration date. Compounding Methods Centrifuge before uncapping to bring contents to the bottom. Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL, which can be diluted even further according to subsequent experiments; dispense as a single experimental dosage and freeze at -20°C ~ -80°C to avoid repeated freezing and thawing. Do not reconstitute directly in cell culture medium. Cautions 1. Please operate with lab coats and disposable gloves,for your safety. 2. This product is for research use only. Documents: Manual 90213_Manual_HB20260828
