The interleukin 17 (IL-17) family of proteins are immunomodulatory cytokines.IL-17E, also known as IL-25, promotes a Th2-favored immune response. This is in contrast to other IL-17 family members, which promote Th1- and Th17-favored inflammation.IL-25 is an important mediator of allergic reactions and protection against intestinal parasites. Mature mouse IL-25 shares 80% and 91% amino acid sequence identity with human and rat IL-25, respectively. IL-25 acts on a variety of cell types that respond with increased production of Th2 cytokines (e.g., IL-4, IL-5, IL-13) and Th1 and Th17 cytokines (e.g., IFN-γ, IL-12, IL-23, IL-17A, IL-17A, and Th17). 23, IL-17A, IL-17F) production is decreased. This product is supplied as a liquid with high activity, high purity, low endotoxin, and NO labeling. Product Properties Synonyms IL25; IL-25; interleukin 25; Interleukin-17E; interleukin-25 Species Mouse Source HEK293 Ceslls Sequence Val17-Ala169 Accession Q8VHC9 Tag NO Predicted molecularmass 17.5 KDa Endotoxin 95%, determined by SDS-PAGE Activity Measured by its ability to induce CXCL1/GRO alpha secretion in HT29 human colon adenocarcinoma cells. The EC50 for this effect is 0.2-1.0 ng/mL. Formulation Dissolved in sterile PBS buffer. Appearance Fluids Shipping and Storage -85~-65℃ storage, valid for 1 year after receipt. Avoid repeated freezing and thawing, centrifuge before opening the vial. It is recommended to aliquot for one experimental use, after aliquoting, freeze at -80℃. Figure Figure 1.Measured by its ability to induce CXCL1/GRO alpha secretion in HT‑29 human colon adenocarcinoma cells. The EC50 for this effect is 0.02-1.0 ng/mL. Figure 2.4 ug/lane protein was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by Coomassie Blue staining. Cautions 1. For your safety and health, please wear lab coat and disposable gloves. 2. This product is for scientific research purposes only.
