Interleukin-18 (IL-18), a pro-inflammatory cytokine belonging to the IL-1 superfamily, is produced by macrophages and other cells. It is expressed as a 24 kDa precursor by endothelial and epithelial cells, keratinocytes, γδT cells and phagocytes. Mature human IL-18 shares approximately 63% amino acid sequence identity with mouse and rat IL-18.Activation of IL-18 is induced by infection or tissue injury and leads to disease pathology in chronic inflammation. In the presence of IL-12 or IL-15, IL-18 enhances antiviral Th1 immune responses by inducing IFN-γ production and cytolytic activity of CD8+ T cells and NK cells.IL-18 induces γδT cells and CD4+ T cells to produce non-antigen-dependent IL-17.IL-18 also promotes maturation of myeloid dendritic cells and triggers a neutrophil Respiratory bursts. In the absence of IL-12 or IL-15, IL-18 promotes the production of Th2 cytokines IL-4 and IL-13 by CD4+ T cells and basophils.IL-18 also promotes maturation of myeloid dendritic cells and triggers a neutrophil respiratory burst. This product Recombinant Human IL-18 is supplied as Lyophilized powder with high activity, high purity, low endotoxin, and NO labeling. Product Properties Synonyms IGIF; IL18; IL-1g; IL1F4 Species Human Source CHO Cells Sequence Tyr37-Asp193 Accession Q14116 Tag NO Molecular Weight Predicted molecularmass：18.2 kDa Endotoxin ≤10 EU/mg by the LAL method Purity ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining) Activity Measured by its ability to induce IFN-gamma secretion by KG1 humanacute myelogenous leukemia cells in the presence of TNF-alpha. The ED50for this effect is ≤2.0 ng/mL. Formulation Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% mannitoland 0.01% Tween 80, pH 7.4 Appearance Lyophilized powder Shipping and Storage -25 ~ -15 ° C storage, from the date of receipt of goods, the expiration date of 1 year. After re-solution, store at 2 ~8 °C, 7-10 days expiration date. After re-dissolution, -85 ~ -65 ° C storage, 6 months expiration date. Compounding method Use NO bacterial deionized water to reconstitute lyophilized proteins. It is recommended that proteins be divided for first use to avoid repeated freeze-thawing. Figure Figure 1.Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED50 for this effect is ≤ 20 ng/mL. Figure 1.Human IL-21 Protein on SDS-PAGE under reducing (R) condition. The purity is greater than 95%. Cautions 1. For your safety and health, please wear lab coat and disposable gloves. 2. This product is for scientific research purposes only.
