IL-1α and IL-1β are potent pro-inflammatory cytokines that induce a wide range of biological activities in various cell types. Two distinct types of IL-1 receptors have been identified and cloned from human and mouse cells. IL-1 Receptor Type I is an 80 kDa transmembrane protein with a well-established role in IL-1 signal transduction. IL-1 Receptor Type II is a 68 kDa membrane protein with a relatively short cytoplasmic tail that lacks signaling capability. The Type II receptor acts as a decoy target for IL-1, inhibiting IL-1 activity by preventing its binding to the Type I receptor. A soluble form of the Type II receptor is induced by anti-inflammatory agents such as glucocorticoids, IL-4, and IL-13. This product is provided as a liquid and features high purity, high bioactivity, and low endotoxin levels. Components Name 90278ES05 90278ES10 90278ES50 90278ES60 90278ES80 Recombinant Human IL-1α Protein (HEK293) 2 μg 10 μg 50 μg(5 ×10 μg) 100 μg 1 mg Specifications Synonyms IL-3, h-IL-3, rh-IL-3, IL-3, interleukin-3, TCGF Species Human Source HEK293 cells Sequence Ser113–Ala271 Accession P01583 Tag No Tag Molecular Weight The protein has a predicted molecular weight of 18.0 kDa Endotoxin < 0.01 EU per μg, as determined by the LAL method Purity > 95%, determined by SDS-PAGE Activity Measured by cell proliferation assay using D10.G4.1 mouse helper T cells. The EC₅₀ for this effect is 1–6 pg/mL Formulation Dissolved in sterile PBS buffer Appearance Liquid Storage Store at –85°C ~ –65°C. The product is stable for one year upon receipt. Avoid repeated freeze-thaw cycles. Centrifuge the vial before opening. It is recommended to aliquot the product into single-use volumes and store the aliquots at –80°C. Figure Figure 1.Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells.The EC50 for this effect is 0.6‑6 pg/mL. Figure 2.4 ug/lane protein was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by Coomassie Blue staining. Documents: Safety Data Sheet 90278_MSDS_HB251015_EN.PDF Manuals 90278_Manual_Ver.EN20251015.pdf
