Recombinant Human sCD40 Ligand Protein, His tag _90606ES

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Description Human sCD40 Ligand, also known as soluble CD40 Ligand protein, is a protein produced by activated T cells. It plays a crucial role in the immune response by binding to its receptor, CD40, which is expressed on the surface of B cells, dendritic cells, and other immune cells. Product information Synonyms CD154 antigen, CD154, CD40 antigen ligand, CD40 Ligand, gp39, HIGM1, T-B cell-activating molecule, T-BAM, TNFSF5, TNFSF5IMD3, TRAP Uniprot No. P29965 Source HEK293 cells-derived human sCD40 Ligand , Met113-Leu261, with N-terminal his tag. Molecular Weight Approximately 17.36 kDa. Purity > 95% as determined by SDS-PAGE. Biological Activity Human CD40 immobilized on CM5 chip can bind human sCD40 Ligand, his tag with an affinity constant of 2.31×10-9 M as determined in a SPR assay (Biacore 8K). Endotoxin < 1 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS. Reconstitution Centrifuge tubes before opening. Dissolve lyophilized protein with PBS. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. 2-7 days, 2 ~8 °C under sterile conditions after reconstitution. 3 months, -25~-15℃ under sterile conditions after reconstitution. Notes 1.Please operate with lab coats and disposable gloves, for your safety. 2.This product is for research use only. Show more