Galectin-3, is classified as a chimeric member of the Galectin superfamily. Nuclear Galectin-3 can modulate gene expression, while cytosolic Galectin-3 can inhibit apoptosis. Galectin-3 contributes to the innate immune response against Candida albicans and Streptococcus pneumoniae, and it can facilitate acute inflammatory responses via neutrophil activation and opsonization, macrophage recruitment, and mast cell activation. Product Properties Synonyms AGE-R3, CBP35, GAL3, L29, LGALS3, Mac-2 Uniprot No. P17931 Source Yeast-derived Human Galectin-3, Ala2-Ile250. Molecular Weight Approximately 26.0 kDa. Purity > 90% by SDS-PAGE. Endotoxin < 1.0 EU per 1μg of the protein by the LAL method. Biological Activity Recombinant human Galectin-3 is able to agglutinate red blood cells at a minimum effective concentration of 25µg/ml. Formulation Lyophilized from a 0.2 µm filtered solution in 1 × PBS, pH 7.4, 1%BSA. Reconstitution 1.We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile PBS, pH 7.4, with 0.1% BSA to a concentration of 0.1-1.0 mg/mL. 2.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in PBS, which must contain carrier proteins, such as 0.1% BSA, 10% FBS, 5% HSA, 5% trehalose, one of four options. Shipping and Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. 1 month, 2 ~8 °C under sterile conditions after reconstitution. 3 months, -25~-15℃ °C under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. 注意事项 1.Please operate with lab coats and disposable gloves,for your safety. 2.This product is for research use only. Cautions Ver. CN2023
