Noggin is a secreted homodimeric glycoprotein that is an antagonist of bone morphogenetic proteins (BMPs). It is very well conserved in vertebrates, so mature human Noggin has high homology to mouse, rat, bovine, equine, and chicken species.Noggin specifically binds and inhibits BMPs, which play an important role in early embryonic development, limb formation, and neurological development, as well as antagonizing BMPs, which affects normal development of the body's bone, cartilage, and joints.Noggin Noggin was originally identified as a BMP-4 antagonist and plays an important role in the proper formation of the head and other dorsal structures. Therefore, Noggin has a high affinity for certain BMPs such as BMP-4, a low affinity for BMP-7, and a high correlation with BMP-2, BMP-13 and BMP-14. During skeletal development, Noggin prevents chondrocyte proliferation, which in turn regulates normal joint formation.Noggin is used in the adult central nervous system and peripheral tissues (e.g., lungs) when human embryonic stem cells (hESCs) or neural stem cells are cultured under certain conditions.Addition of Noggin to antagonize BMP activity results in the proliferation of the stem cells, while keeping them in an undifferentiated state, or differentiating them into dopaminergic neurons. In Noggin-deficient mice, enhanced BMP activity causes a range of developmental abnormalities, including failure of neural tube formation, delayed hair follicle development, axial skeletal malformations, and arthropathy. This recombinant mouse Noggin is high activity, high purity, low endotoxin and label free. Specification Synonyms Noggin; NOG; SYM1; SYNS1 Source HEK293 Sequence Leu20-Cys232 Accession P97466 Molecular Weight Predicted Moleucular weight: 23.1 kDa Endotoxin < 0.01 EU/μg by the LAL method. Purity > 95%，determined by SDS-PAGE Biological Activity Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The EC50 for this effect is 12-20 ng/mL in the presence of 50 ng/mL of Recombinant Human BMP-4. Formulation Dissolved in sterile PBS buffer. Tag Not Physical Appearance Fluids Storage -85~-65℃ storage, valid for 1 year after receipt. Avoid repeated freezing and thawing, centrifuge before opening the vial. It is recommended to aliquot for one experimental use, after aliquoting, freeze at -80℃. Cautions 1. For your safety and health, please wear lab coat and disposable gloves. 2. This product is for scientific research purposes only.
