THP-1 cells are a human monocytic leukemia cell line and a classic in vitro model for studying macrophage function. In experiments, cytokine stimulation is a common method for inducing THP-1 cell differentiation. Cytokines such as IL-4 and IL-13 can promote the differentiation of THP-1 cells into macrophages and initiate specific signaling pathways, thereby regulating cell function. This method can simulate in vivo immunomodulatory processes and provide a convenient way to study the functions of macrophages in inflammatory responses, immunomodulation, and anti-tumor activities. Macrophages are classified into two main types: M1-type macrophages and M2-type macrophages. THP-1 cells are usually induced to differentiate into macrophages using phorbol ester (PMA), and then, in the continued presence of PMA: lipopolysaccharide (LPS) and IFN-γ are added and cultured for 48 hours to induce M1 polarization; IL-4 and IL-13 are added and cultured for 48 hours to induce M2 polarization. Finally, the cells are resuspended in serum-free RPMI-1640 basal medium without stimulants and PMA, and can be maintained for 72 hours. This kit is used to differentiate THP-1 cells into macrophages (M1 or M2 type). This kit contains five reagents: recombinant human IL-13, recombinant human IL-4, recombinant human IFN-γ, phorbol ester (PMA), and lipopolysaccharide (LPS). PMA is used to initially differentiate THP-1 monocytes into unpolarized M0 macrophages. IFN-γ and LPS initiate the core signals for M1 polarization. IL-4 and IL-13 act synergistically to drive cell polarization towards the M2 phenotype. Product Information Product Information. Cat. NO. Size Human THP-1 Cell Polarization Induction Kit 92640ES08 5 μg 92640ES50 50 μg 92640ES60 100 μg Components Component Cat No. Component Name 92640ES08 92640ES50 92640ES60 92640-A Human IL-4 Protein 5 μg 50 μg 100 μg 92640-B Human IL-13 Protein 10 μg 50 μg 100 μg 92640-C Human IFN-γ Protein 5 μg 50 μg 100 μg 92640-D Lipopolysaccharide (LPS) 5 mg 5 mg 5 mg 92640-E PMA 1 mg 1 mg 1 mg Product Includes Component Cat No. Product Name Source Appearance Quantity 90105ES Human IL-4 Protein E.coli Lyophilized Powder 1 90112ES Human IL-13 Protein E.coli Lyophilized Powder 1 91207ES Human IFN-γ Protein E.coli Lyophilized Powder 1 60747ES Lipopolysaccharide (LPS) E.coli Lyophilized Powder 1 50601ES PMA Plant Powder 1 Reconstitution Method Component Name Reconstitution Method Human IL-4 Protein Before opening the cap, centrifuge first to bring the contents to the bottom. Reconstitute using sterile water to a concentration of 0.1-1.0 mg/mL. It can be further diluted according to subsequent experiments. For long-term storage, it is recommended to add carrier protein (0.1% BSA) to the dilution buffer; aliquot into single-use amounts, store at -80°C, and avoid repeated freeze-thaw cycles. Human IL-13 Protein Human IFN-γ Protein Lipopolysaccharide (LPS) Reconstitute with water or cell culture medium to 1 mg/mL. PMA Dissolve in DMSO or Ethanol Storage Component Name Storage Conditions Human IL-4 Protein Store at -25 ~ -15°C. Valid for 1 year from the date of receipt. After reconstitution, store at 2~8°C under sterile conditions, valid for 7 days. After reconstitution, store at -85 ~ -65°C under sterile conditions, valid for 3 months. Human IL-13 Protein Human IFN-γ Protein Lipopolysaccharide (LPS) Store at 2~8°C protected from light, valid for 6 years. PMA Store at -25~-15°C protected from light, valid for 3 years. Notes 1. For your safety and health, please wear a lab coat and disposable gloves during operation. 2. This product is for research use only. Documents Manuals: 92640_Manual_HB20260825 Related Products Cat.NO. Product Name 92639ES Human Organoids Cytokine Set 92641ES Mouse BMDC Differentiation Cytokine Set 92643ES Mouse Macrophage BMDM Cytokine Set 92644ES Human Dendritic Cell Differentiation Cytokine Set
