This kit contains recombinant mouse IL-6, mouse TGF-β1, mouse IL-23, and mouse IL-1β, and is used to induce the differentiation of mouse naïve CD4+ T cells into Th17 cells. IL-6 and TGF-β1 act synergistically to activate the STAT3 signaling pathway. This is essential for overcoming TGF-β-induced Treg differentiation and shifting towards the Th17 differentiation program. They also induce the production of the autocrine factor IL-21, which further amplifies the Th17 response. IL-23 is added during the middle to late stages of Th17 differentiation. By activating STAT3, it maintains the expression of RORγt and the IL-23 receptor, promotes the terminal differentiation and expansion of Th17 cells, and enhances their pathogenicity. In the absence of IL-23, the resulting Th17 cells may be unstable and prone to convert into other subtypes. IL-1β is a potent enhancer. For mouse Th17 cells, adding IL-1β can significantly improve polarization efficiency (the proportion of IL-17A+ cells), especially when using serum-free media or when polarization efficiency is suboptimal. These cytokines constitute the "instructive" microenvironment for Th17 differentiation and are key to the success of the experiment. Product Information Product Information. Catalog Number Size Mouse Th17 Cell Polarization Cytokine Set 92654ES10 10 μg 92654ES50 50 μg 92654ES60 100 μg Components Component Cat No. Component Name 92654ES10 92654ES50 92654ES60 Quantity 92654ES-A Mouse IL-6 Protein 10 μg 50 μg 100 μg 1 92654ES-B Mouse TGF-β1 Protein 10 μg 50 μg 100 μg 1 92654ES-C Mouse IL-23 Protein 10 μg 50 μg 100 μg 1 92654ES-D Mouse IL-1β Protein 10 μg 50 μg 100 μg 1 Product Includes Component Cat No. Product Name Source Appearance 90146ES Recombinant Mouse IL-6 Protein E.coli Lyophilized powder 91701ES Recombinant Mouse TGF-β1 Protein HEK293 Liquid 90226ES Recombinant Mouse IL-23 Protein HEK293 Lyophilized powder 90140ES Recombinant Mouse IL-1β Protein E.coli Lyophilized powder Reconstitution Method Component Name Reconstitution Method Recombinant Mouse IL-6 Protein Before opening, centrifuge first to bring the contents to the bottom. Reconstitute with sterile deionized water to a concentration of 0.1-1.0 mg/mL. It can be further diluted according to subsequent experiments. For long-term storage, it is recommended to add carrier protein (0.1% BSA) to the dilution buffer; aliquot into single-use amounts, store frozen at -80°C, and avoid repeated freeze-thaw cycles. Recombinant Mouse IL-23 Protein Recombinant Mouse IL-1β Protein Performance Parameters Species Mouse Tag No Tag Expression System HEK293 Purity > 95% as determined by SDS-PAGE. Endotoxin < 0.01 EU per µg by the LAL method. Appearance Lyophilized Powder Storage Store at -85 ~ -65°C. Valid for 1 year from the date of receipt.After reconstitution, store at -85 ~ -65°C under sterile conditions for 3 months. Product Features High activity, high purity, high stability, low endotoxin Documents Manual 92654_Manual_HB20260409
