This kit contains recombinant mouse IL-2, IL-4, IL-21, and BAFF for the in vitro polarization of mouse B cells. IL-2 is typically added as an enhancing component to existing polarization systems. Its most important applications include synergizing with IL-4 to enhance IgG1/IgE class switching, and with TGF-β to enhance IgA class switching. In the early stages of the B cell response, it promotes clonal expansion and prepares cells for class switching; in later stages, it may influence the fate decision between plasma cells and memory B cells. IL-4 is the core instructive factor for IgG1 and IgE class switching. In T cell-dependent responses, IL-4 is the most critical cytokine for inducing B cells to switch from expressing IgM/IgD to expressing IgG1 and IgE. Meanwhile, it is a potent B cell co-stimulatory proliferation factor, acting synergistically with BCR or CD40 signaling. IL-21 induces B cell differentiation into plasma cells and antibody secretion. BAFF promotes B cell survival and maturation. Product Information Product Information. Catalog Number Size Mouse B lymphocyte Polarization Cytokine Set 92655ES10 10 μg 92655ES50 50 μg 92655ES60 100 μg Components Component Cat No. Component Name 92655ES10 92655ES50 92655ES60 Quantity 92655ES-A Mouse IL-2 Protein 10 μg 50 μg 100 μg 1 92655ES-B Mouse IL-4 Protein 10 μg 50 μg 100 μg 1 92655ES-C Mouse IL-21 Protein 10 μg 50 μg 100 μg 1 92655ES-D Mouse BAFF Protein 10 μg 50 μg 100 μg 1 Product Includes Component Cat No. Product Name Source Appearance 90239ES Mouse IL-2 Protein HEK293 Liquid 90241ES Mouse IL-4 Protein HEK293 Liquid 90233ES Mouse IL-21 Protein HEK293 Liquid 90641ES Mouse BAFF Protein HEK293 Lyophilized powder Reconstitution Method Component Name Reconstitution Method Recombinant Mouse BAFF Protein Before opening, centrifuge to bring the contents to the bottom. Reconstitute with ultrapure water to a concentration of 0.1-1.0 mg/mL. Further dilution can be performed based on subsequent experiments. Performance Parameters Species Mouse Tag No Tag Expression System HEK293 Purity > 95% as determined by SDS-PAGE. Endotoxin < 0.01 EU per µg by the LAL method. Formulation PBS buffer. Appearance Liquid Storage Store at -85 ~ -65°C. Valid for 1 year from the date of receipt.After reconstitution, store at -85 ~ -65°C under sterile conditions for 3 months. Product Features High activity, high purity, high stability, low endotoxin. Documents Manual 92655_Manual_HB20260409
