Human NKG2D/CD314 (His-Avi Tag)

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NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. Human NKG2D is expressed on CD8+ alpha beta T cells, gamma δ T cells, NK cells and NKT cel ls. In mouse systems NKG2D also occurs on macrophages. Human ligands for NKG2D include MICA, MICB, and ULBP1, 2, and 3. Expression of NKG2D ligands occurs in epithelial cells, tumor cells and under conditions of stress or infection. NKG2D exists as a disulfide-linked homodimer that delivers an activating signal upon ligand binding. NKG2D has been implicated in anti-tumor surveillance and the immune response against viral infection. Specifications Synonyms CD314, D12S2489E, KLR, NKG2-D, NKG2D Uniprot No. P26718 Source Recombinant Human NKG2D / CD314 Protein is expressed from HEK293 Cells with His tag and Avi tag at the N-terminal. It contains Phe78-Val216. Molecular Weight Approximately 19 kDa. Due to glycosylation, the protein migrates to 36-38 kDa based on Tris-Bis PAGE result. Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGE Activity ELISA Data: Immobilized Human NKG2D, His Tag at 1 μg/mL (100 μL/well) on the plate. Dose response curve for Anti-NKG2D Antibody, hFc Tag with the EC50 of 4.7 ng/mL determined by ELISA. Endotoxin < 1.0 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. Storage The product should be stored at -20~-80℃ for 1 year from date of receipt. 2-7 days, 2 ~8 °C under sterile conditions after reconstitution. 3-6 months, -20~-80℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Note 1. Avoid repeated freezing and thawing. 2. Please operate with lab coats and disposable gloves, for your safety. 3. This product is for research use only. Product Data Show more