Mesothelin, also known as CAK1 and ERC, is derived from a 70 kDa precursor that also includes Megakaryocyte Potentiating Factor (MPF). MPF is a cytokine that potentiates IL-3 induced megakaryocyte colony formation. This recombinant human Mesothelin lacks the 8 aa insertion, and within aa 296-580 it shares 59% sequence identity with mouse and rat Mesothelin. Mesothelin is normally expressed on mesothelial cells in the pleura, pericardium, and peritoneum as well as in the developing and postnatal pancreas. It is upregulated in mesotheliomas and a range of carcinomas and adenomas. Mesothelin promotes tumor cell proliferation, migration, anchorage-independent growth, and tumor progression. Specifications Synonyms MSLN; CAK1; Mesothelin; MPF; MPFSMRP; SMR Uniprot No. Q13421.2 Source Recombinant Biotinylated Human MSLN/Mesothelin Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Glu296-Gly580. Molecular Weight Approximately 35.2 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Biotinylated Human MSLN at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-MSLN Antibody, hFc Tag with the EC50 of 20.3ng/ml determined by ELISA. Endotoxin < 1.0 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. Storage The product should be stored at -20~-80℃ for 1 year from date of receipt. 2-7 days, 2 ~8 °C under sterile conditions after reconstitution. 3-6 months, -20~-80℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Note 1. Avoid repeated freezing and thawing. 2. Please operate with lab coats and disposable gloves, for your safety. 3. This product is for research use only. Product Data Figure 1. The purity of Biotinylated Human MSLN wasgreater than 95% as determined by Tris-Bis PAGE and SEC-HPLC. Figure 2. Immobilized Biotinylated Human MSLNat 0.5μg/ml (100μl/well) on the plate. Dose responsecurve for Anti-MSLN Antibody, hFc Tag with the EC50 of 20.3ng/ml determined by ELISA.
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