Human CLEC12A/MICL/CLL-1 (His Tag)

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C-type lectin domain family 12 member A (CLEC12A), also known as C-type lectin-like molecule 1 (CLL1), dendritic cell-associated lectin 2 (DCAL-2), myeloid inhibitory C-type lectin -like receptor (MICL), killer cell lectin like receptor-1 (KLRL1) and CD371, is a member of the C-type lectin receptor superfamily. C-type lectins are the most diverse and prevalent lectin family in immunity. Particular interest has recently been attracted by the C-type lectin-like receptors on NK cells, which appear to regulate the activation/inhibitory balance of these cells, controlling cytotoxicity and cytokine production. CLEC12A receptor has emerged as a leukemia-associated and cancer stem cell marker in myeloid malignancies. CLEC12A is a myeloid lineage antigen that is highly expressed by AML cells and LSCs, but not expressed by normal hematopoietic stem cells (HSCs). The CLEC12A TriKE induced robust NK-cell specific proliferation, enhanced NK-cell activation, and killing of both AML cell lines and primary patient-derived AML blasts in vitro while sparing healthy HSCs. Specifications Synonyms MICL; CLL-1; CLEC12A; CLL1; DCAL2; DCAL-2; CD371; CD303; CLECSF11; CLECSF7; DLEC; HECL; PRO34150; DCAL-2 Uniprot No. Q5QGZ9.2 Source Recombinant Human CLEC12A/MICL/CLL-1 Protein is expressed from HEK293 Cells with His tag at the N-terminal. It contains His65-Ala265. Molecular Weight Approximately 24.6 kDa. Due to glycosylation, the protein migrates to 38-55 kDa based on Tris-Bis PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data：Immobilized Human CLEC12A at 0.2μg (100μl/Well) on the plate. Dose response curve for Anti-CLEC12A Antibody, hFc Tag with the EC50 of 7.9ng/ml determined by ELISA. Endotoxin < 1.0 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. Storage The product should be stored at -20~-80℃ for 1 year from date of receipt. 2-7 days, 2 ~8 °C under sterile conditions after reconstitution. 3-6 months, -20~-80℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Note 1.Avoid repeated freezing and thawing. 2.Please operate with lab coats and disposable gloves, for your safety. 3.This product is for research use only. Product Data Show more