Specifications Synonyms SIMP; FAPalpha;FAP; APCE Source Mouse FAP Protein is expressed from HEK293 with His tag at the N-Terminus. It contains Leu26-Asp761.[Accession | P97321-1] Molecular Weight The protein has a predicted MW of 86.4 kDa. Due to glycosylation, the protein migrates to 90-100 kDa based on SDS-PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 95% as determined by SDS-PAGE Activity ELISA Data: Immobilized Mouse FAP, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA . Bioactivity Data： Measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC). The specific activity is >2000 pmol/min/µg Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. 2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution. 3 -6 months, -85~-65℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Note 1.Please operate with lab coats and disposable gloves, for your safety. 2.This product is for research use only.
