Specifications Source Recombinant Cynomolgus Nectin-4 Protein is expressed from HEK293 with His tag at the C-terminal. It contains Gly32-Val351.[Uniprot | XP_005541277.1] Molecular Weight The protein has a predicted MW of 35.9 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Tris-Bis PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 95% as determined by Tris-Bis PAGE and HPLC Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. 2-7 days, 2 ~8 °C under sterile conditions after reconstitution. 3 months, -25~-15℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Note 1.Please operate with lab coats and disposable gloves,for your safety. 2.This product is for research use only. Product Data ELISA Data Figure 1.Immobilized Cynomolgus Nectin-4, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Nectin-4 Antibody, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA.
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