Human CD30 Ligand/TNFSF8 Protein, His Tag

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Specifications Source Human CD30 Ligand/TNFSF8 Protein is expressed from HEK293 with His tag at the N-terminal. It contains Gln63-Asp234.[Accession | P32971] Molecular Weight The p rotein has a predicted MW of 20.7 kDa. Due to glycosylation, the protein migrates to 30-50 kDa based on SDS- PAGE result. Endotoxin 95% as determined by SDS- PAGE and HPLC Activity ELISA Data: Immobilized Human CD30 Ligand, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human CD30, hFc Tag with the EC50 of 8.9ng/ml determined by ELISA. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. 2-7 days, 2 ~8 °C under sterile conditions after reconstitution. 3 months, -25~-15℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Note 1.Please operate with lab coats and disposable gloves,for your safety. 2.This product is for research use only. Show more