Specifications Source Recombinant Human VEGF R3/FLT4 Protein is expressed from HEK293 with hFc tag at the C-terminal. It contains Tyr25-Ile776.[ Uniprot | P35916-1] Molecular Weight The protein has a predicted MW of 111.3 kDa. Due to glycosylation, the protein migrates to 135-155 kDa based on Tris-Bis PAGE result. Endotoxin Less than 1EU per ug by the LAL method. Purity > 95% as determined by Tris-Bis PAGE and HPLC Activity ELISA Data: Immobilized Human VEGF-C, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human VEGFR3, hFc Tag with the EC50 of 19.8ng/ml determined by ELISA. Formulation Lyophilized from 0.22 μm filtered solution in 50mM Tris, 150mM NaCl, 100mM Glycine (pH 7.5). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. 2-7 days, 2 ~8 °C under sterile conditions after reconstitution. 3 months, -25~-15℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Note 1.Please operate with lab coats and disposable gloves,for your safety. 2.This product is for research use only.
