Specifications Synonyms Dkk-1; Dickkopf-1;Dickkopf-related 1; hDkk-1 Source Biotinylated Human DKK1 C terminal Domain Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus. It contains Met178-His266.[Accession | O94907] Molecular Weight The protein has a predicted MW of 38.69 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on SDS-PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 95% as determined by SDS-PAGE Activity ELISA Data: Immobilized Anti-DKK1 Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human DKK1 C terminal Domain, hFc Tag with the EC50 of 19.7ng/ml determined by ELISA .Immobilized Human LRP-6, mFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human DKK1 C terminal Domain, hFc Tag with the EC50 of 0.85ug/ml determined by ELISA. Formulation Lyophilized from 0.22μm filtered solution in 20mM NaAc, 150mM NaCl (pH 5.0). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 20mM NaAc, 150mM NaCl (pH 5.0). Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. 2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution. 3 -6 months, -85~-65℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Note 1.Please operate with lab coats and disposable gloves for your safety. 2.This product is for research use only.
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