Specifications Source Recombinant Biotinylated Human CD24 Protein (Primary Amine Labeling) is expressed from HEK293 with mFc (IgG1) tag at the C-terminal. It contains Ser27-Gly59.[uniprot | P25063-1] Molecular Weight The protein has a predicted MW of 29.5 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Tris-Bis PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 95% as determined by Tris-Bis PAGE and HPLC Activity ELISA Data: Immobilized Anti-CD24 Antibody at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human CD24, mFc Tag with the EC50 of 0.83μg/ml determined by ELISA. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. 2-7 days, 2 ~8 °C under sterile conditions after reconstitution. 3 months, -25~-15℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Note 1.Please operate with lab coats and disposable gloves,for your safety. 2.This product is for research use only.
