Specifications Source Recombinant Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein is expressed from HEK293 with hFc tag at the C-terminal. It contains Asp23-Asp126 (CD3E)&Gln23-Ser116(CD3G).[uniprot | P07766(CD3E)&P09693(CD3G)] Molecular Weight The protein has a predicted MW of 37.8 kDa (CD3E) and 36.5 kDa (CD3G). Due to glycosylation, the protein migrates to 40-60 kDa based on Tris-Bis PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 95% as determined by Tris-Bis PAGE and HPLC Activity ELISA Data: Immobilized Human CD3E&CD3G, hFc Tag at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Anti-CD3E&CD3G Ab.1, hFc Tag with the EC50 of 1.22ug/ml determined by ELISA. ELISA Data: Immobilized Human CD3E&CD3G, hFc Tag at 1μg/ml (100μl/Well). Dose response curve for Anti-CD3E&CD3G Ab.2, mFc Tag with the EC50 of 20.7ng/ml determined by ELISA Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. 2-7 days, 2 ~8 °C under sterile conditions after reconstitution. 3 months, -25~-15℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Note 1.Please operate with lab coats and disposable gloves,for your safety. 2.This product is for research use only.
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