Specifications Synonyms CD172a; MYD1; SHPS-1; SIRP alpha; SIRPalpha2 Source Human SIRP alpha V2/CD172a Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Glu31-Arg369.[Accession | AAH26692.1] Molecular Weight The protein has a predicted MW of 38 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on SDS-PAGE result. Endotoxin < 1EU per μg by the LAL method. Activity Blocking Data: Serial dilutions of Anti-SIRP alpha Antibody, hFc Tag were added into Human SIRP alpha V2, His Tag : Biotinylated Human CD47 Protein, His Tag binding reactioins.The half maximal inhibitiory concentration (IC50) is 54.14ng/ml. ELISA Data: Immobilized Human SIRP alpha V2, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-SIRP alpha Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA.Immobilized Human SIRP alpha V2, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 0.15μg/ml determined by ELISA. SPR Data: Human SIRP alpha V2, His Tag captured on Protein A Chip via Anti-His Antibody can bind Anti-SIRP alpha Ab., hFc Tag with an affinity constant of 9.11nM as determined in SPR assay . Purity > 95% as determined by SDS-PAGE and by HPLC. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. 2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution. 3-6 months, -85~-65℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Note 1. Please operate with lab coats and disposable gloves for your safety. 2. This product is for research use only.
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