ApoE is a major protein component of serum LDL, VLDL, HDL, and chylomicrons. It is produced predominantly by hepatocytes, macrophages, and non-neuronal cells in the CNS. ApoE-containing particles transport triglycerides and cholesterol to peripheral tissues for cellular uptake and catabolism. Mature human ApoE is a 37 kDa glycoprotein. ApoE3 is the most abundant of the three common alleles in human; ApoE2 and ApoE4 differ by single aa substitutions. Mature human ApoE shares 71% aa sequence identity with mouse and rat ApoE. ApoE3 dampens the TNF-alpha induced inflammatory response in vascular endothelial cells. Specifications Synonyms Apolipoprotein E, Apo-E, APOE, apolipo E Uniprot No. P02649-1 Source Recombinant Human APOE / Apolipoprotein E Protein is expressed from HEK293 Cells with His tag at the N-terminal. It contains Lys19-His317. Molecular Weight Approximately 35.3 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result. Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGE Activity 1. SPR Data: Human TREM2, hFc Tag captured on CM5 Chip via Protein A can bind Human APOE, His Tag with an affinity constant of 1.004 μM as determined in SPR assay (Biacore T200). 2. SPR Data: Human LILRB4, hFc Tag captured on CM5 Chip via Protein A can bind Human APOE, His Tag with an affinity constant of 115.9 nM as determined in SPR assay (Biacore T200). Endotoxin < 1.0 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. Storage Transported with ice packs. Store at -20℃ to -80℃, valid for one year. After reconstitution, store unopened at -20 to -80°C for 3-6 months. After reconstitution, store at 2-8°C for 2-7 days. It is recommended to store in aliquots for first use to avoid repeated freezing and thawing. Note 1. Avoid repeated freezing and thawing. 2. For your safety and health, please wear a lab coat and disposable gloves when operating. 3. This product is for scientific research purposes only! Product Data
