Tumor Necrosis Factor Receptor II (TNF RII), also known as TNFRSF1B, p75/p80, and CD120b, is a type I transmembrane protein that belongs to the TNF receptor superfamily. It has a molecular weight of approximately 75 kDa. Human TNF RII contains four cysteine-rich repeats in its ECD, which shares 58% and 56% amino acid sequence identity with the mouse and rat orthologs, respectively. Those receptors that lack DDs, like TNF RII, bind TNF Receptor-associated Factors, which transduce signals generated by activation of these receptors. Polymorphisms of the human TNFR2 gene, which result in increased expression of both membrane-bound and soluble TNF RII, have been associated with several autoimmune diseases including Crohn’s disease, systemic lupus erythematosus, and familial rheumatoid arthritis. Specifications Synonyms CD120b, Etanercept, p75TBPII, p75TNFR, TNF RII, TNF-R2, TNF-R75, TNFR80, TNFRSF1B, TNFR2, TBPII, TNFBR, TNFR1B Uniprot No. P20333-1 Source Recombinant Human TNFR2 / CD120b / TNFRSF1B Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains Leu23-Asp257. Molecular Weight Approximately 26.2 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on Tris-Bis PAGE result. Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGE> 90% as determined by HPLC Activity 1. ELISA Data: Immobilized Human TNFR2, His Tag at 0.5 μg/mL (100 μL/Well). Dose response curve for Biotinylated Human TNF alpha, His Tag with the EC50 of 16.6 ng/mL determined by ELISA. 2. ELISA Data: Immobilized Human TNFR2, His Tag at 0.5 μg/mL (100 μL/Well). Dose response curve for Anti-TNFR2 Antibody, hFc Tag with the EC50 of 12.7 ng/mL determined by ELISA. Endotoxin < 1.0 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Storage Transport with ice packs. Store at -20℃ to -80℃, valid for one year. Once reconstituted, store unopened at -20 to -80°C for 3-6 months. After reconstitution, store at 2-8°C for 2-7 days. It is recommended to store in aliquots and freeze for the first use to avoid repeated freezing and thawing. Note 1. Avoid repeated freezing and thawing. 2. For your safety and health, please wear a lab coat and disposable gloves when operating. 3. This product is for scientific research purposes only. Product Data Tris-Bis PAGE Human TNFR2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. The purity of Human TNFR2 is greater than 90% as determined by SEC-HPLC. ELISA Data Immobilized Human TNFR2, His Tag at 0.5 μg/mL (100 μL/Well). Dose response curve for Biotinylated Human TNF alpha, His Tag with the EC50 of 16.6 ng/mL determined by ELISA. ELISA Data Immobilized Human TNFR2, His Tag at 0.5 μg/mL (100 μL/Well). Dose response curve for Anti-TNFR2 Antibody, hFc Tag with the EC50 of 12.7 ng/mL determined by ELISA.
