T cell surface antigen CD2 is a type I glycoprotein belonging to the Ig superfamily and is expressed on T cells, NK cells, B cells and some antigen presenting cells. CD2 functions as an activator of T lymphocytes and thymocytes. Mature human CD2 consists of a 185 amino acid (aa) extracellular domain (ECD), a 26 aa transmembrane segment, and a 116 aa cytoplasmic tail. The extracellular domain is composed of two immunoglobulin-superfamily domains with highly-charged binding regions. Within the ECD, human CD2 shares 47% and 48% aa sequence identity with mouse and rat CD2, respectively. It interacts with both CD58 and CD59 directly to activate T cells and their adhesion pathways. The conformational flexibility of the CD2 molecule affects function through the conformational status of the adhesive ligands. Together with PSTPIP1, CD2 works to regulate T cell activation. These two proteins colocalize with a second CD2-binding protein to signal immunological synapse formation in T cells. CD2 is an adhesion molecule present on the cell surface of T cells, natural killer (NK) cells, and B cells; and its interaction with CD58 on antigen-presenting cells plays an important role in their immune reaction. CD2-CD58 interactions play a critical role in the anti-tumor immune response, and restoration of this signaling is an important strategy for anti-tumor therapy. Specifications Synonyms T11; CD2; SRBC; LFA-2; LFA-3 receptor Uniprot No. P06729 Source Recombinant Human CD2/SRBC Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains Lys25-Asp209. Molecular Weight The protein has a predicted MW of 22.3 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result. Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGE and HPLC Activity SPR Data：Human CD58, hFc Tag captured on CM5 Chip via Protein A can bind Human CD2, His Tag with an affinity constant of 440 nM as determined in SPR assay (Biacore T200). Endotoxin < 1.0 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. Storage Transported with ice packs. Store at -20℃ to -80℃, valid for one year. After reconstitution, store unopened at -20 to -80°C for 3 to 6 months. After reconstitution, store at 2 to 8°C for 2 to 7 days.。 It is recommended to store in aliquots and freeze for the first use to avoid repeated freezing and thawing. Note 1. Avoid repeated freezing and thawing. 2. For your safety and health, please wear a lab coat and disposable gloves when operating. 3. This product is for scientific research purposes only. Product Data Tris-Bis PAGE
