Fas (fibroblast associated; also known as APO-1 or CD95) is a member of the death receptor subfamily of the TNF receptor superfamily and is designated TNFRSF6. The human Fas precursor is 335 aa in length, and contains a 25 aa signal peptide, a 148 aa extracellular domain (ECD), a 17 aa transmembrane sequence, and a 145 aa cytoplasmic region. The ECD possesses three cysteine-rich TNFR repeats, while the cytoplasmic region contains one death domain (DD) that is required for the transduction of apoptotic signals (4). Human Fas ECD shares 55%, 58% 62% 63% and 64% aa sequence identity with mouse, rat, feline, bovine and porcine Fas ECD, respectively. A human Fas isoform of 314 aa that lacks the transmembrane sequence is secreted by resting lymphocytes, while isoforms of 149, 132, 103 and 86 aa that also lack the DD and show substitutions for parts of the TNFR repeats are less prominently expressed. All five isoforms block the extrinsic apoptosis pathway induced by Fas ligand binding. Fas ligand (FasL; also TNFSF6) is a type II transmembrane protein that belongs to the TNF family and is expressed on activated T-cells, NK cells, and cells found in immune privileged sites. Alternatively, FasL is also shed as a soluble form. Engagement of Fas induces oligomerization of preformed Fas trimers. This activated receptor complex recruits the adaptor molecule FADD to form the Death-Inducing Signaling Complex (DISC). Upon activation, caspases in the DISC initiate the apoptotic signaling cascade. Fas is prominent in epithelial cells, hepatocytes, activated mature lymphocytes, virus-transformed lymphocytes and tumor cells. It is an essential mediator in the activation-induced death of T lymphocytes that terminates the immune reaction. Specifications Synonyms FASLG receptor; CD95; APT1; FAS1; TNFRSF6; FAS; ALPS1A; APO1; FASTM; FasR Uniprot No. P25445-1 Source Recombinant Human Fas/TNFRSF6/CD95 Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains Gln26-Asn173. Molecular Weight The protein has a predicted MW of 13.7 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result. Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGE Activity SPR Data：Human Fas, His Tag immobilized on CM5 Chip can bind Human Fas Ligand, His Tag with an affinity constant of 19.45 nM as determined in SPR assay. Endotoxin < 1.0 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. Storage Transported with ice packs. Store at -20℃ to -80℃, valid for one year. After reconstitution, store unopened at -20 to -80°C for 3 to 6 months. After reconstitution, store at 2 to 8°C for 2 to 7 days.。 It is recommended to store in aliquots and freeze for the first use to avoid repeated freezing and thawing. Note 1. Avoid repeated freezing and thawing. 2. For your safety and health, please wear a lab coat and disposable gloves when operating. 3. This product is for scientific research purposes only. Product Data Tris-Bis PAGE
