C1q R1, also known as CD93 and C1q Rp, is an approximately 125 kDa transmembrane glycoprotein that is involved in various aspects of inflammatory reactions. Mature human CD93 consists of a 557 amino acid (aa) extracellular domain (ECD) with one Ctype lectin domain, four tandem EGFlike domains, and a mucinlike domain, followed by a 21 aa transmembrane segment and a 51 aa cytoplasmic domain. It is distinct from the 60 kDa Calreticulin which is likewise known as C1q R1. Unlike Calreticulin, and despite its name, C1q R1/CD93 does not appear to bind the complement protein C1q. CD93 is expressed by vascular endothelial cells and by a variety of hematopoietic cells. Various sized fragments of soluble CD93 (5075 kDa) can be shed from monocytes, neutrophils, and vascular endothelial cells following inflammatory stimulation, leaving a residual stub in the membrane. Crosslinking of cell surface CD93 enhances phagocytosis by monocytes and enhances the uptake of apoptotic cells in vivo. Soluble CD93 promotes the differentiation of monocytes to macrophages, phagocytosis of apoptotic cells, and inflammatory responsiveness to multiple TLR ligands . It is required for plasma cell longevity in the bone marrow and for the resulting extended humoral response. Specifications Synonyms C1q/MBL/SPA receptor; C1qR; C1qR(p); C1qRp; CDw93; CD93; C1QR1; MXRA4; ECSM3; dJ737E23.1 Uniprot No. Q9NPY3 Source Recombinant Human CD93/C1q R1 Protein is expressed from HEK293 Cells with hFc tag at the C-terminal. It contains Thr22-Lys580. Molecular Weight Approximately 85 kDa. Due to glycosylation, the protein migrates to 115-120 kDa based on Tris-Bis PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human IGFBP-7, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human CD93, hFc Tag with the EC50 of 2.6μg/ml determined by ELISA. Endotoxin < 1.0 EU per 1μg of the protein by the LAL method. Formulation Supplied as 0.22μm filtered solution in PBS (pH 7.4). Please dilute to the desired concentration according to the concentration of the solution shown on the product label. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. Storage Transported with ice packs. Store at -20℃ to -80℃, valid for one year. After reconstitution, store unopened at -20 to -80°C for 3 to 6 months. After reconstitution, store at 2 to 8°C for 2 to 7 days.。 It is recommended to store in aliquots and freeze for the first use to avoid repeated freezing and thawing. Note 1. Avoid repeated freezing and thawing. 2. For your safety and health, please wear a lab coat and disposable gloves when operating. 3. This product is for scientific research purposes only. Product Data
