Specifications Synonyms GDF-15; MIC-1; NAG-1; PDF; PLAB; PTGFB; GDF15; MIC1; RG-1; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta; Species Human Source E.coli Sequence Ala197-Ile308 Accession Q99988 Tag N-His Molecular Weight The protein has a predicted MW of 13.5 kDa. The protein migrates to 15-16 kDa based on Bis-Tris PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 95% as determined by Bis-Tris PAGE Activity ELISA Data: Immobilized Human GDF15, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 11.5ng/ml determined by ELISA. Blocking Data: Serial dilutions of Anti-GDF15 Antibody were added into Human GDF15, His Tag : Biotinylated Human GFRAL, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 40.8ng/ml. SPR Data: Human GFRAL, hFc Tag captured on CM5 Chip via Protein A can bind Human GDF15, His Tag with an affinity constant of 0.014 nM as determined in SPR assay . Formulation Lyophilized from 0.22μm filtered solution in 50mM HAc (pH 2.9). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized powder Storage Store at -25~-15℃, valid for 1 year after receipt. After reconstitution, store at -85~-65℃, valid for 3 months. Reconstitution method Centrifuge before opening the lid. Recommended reconstitution concentration is above 100 μg/ml. Dissolve the lyophilized protein in 50 mM HAC (pH 2.9). Note 1. For your safety and health, please wear a lab coat and disposable gloves when operating. 2. This product is for scientific research purposes only.
