Human IL-15RA&IL-15 Protein, His Tag _ 93831ES

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Specification Source Human IL-15RA&IL-15 Protein is expressed from HEK293 with His tag at the C-terminal. It contains Ile31-Ser108(IL-15RA)&Asn49-Ser162(IL-15).[Accession | Q13261- 1&P40933-1] Molecular Weight The protein has a predicted MW of 23.8 kDa. Due to glycosylation, the protein migrates to 26-28 kDa&30-32 kDa based on Tris-Bis PAGE result. Endotoxin Less than 1EU per ug by the LAL method. Purity > 95% as determined by Tris-Bis PAGE and HPLC Activity ELISA Data: Immobilized Human IL-15RA&IL-15, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human IL-2 R beta&IL-2 R gamma, hFc Tag with the EC50 of 72.0 ng/ml determined by ELISA SPR Data: Human IL-2 R beta&IL-2 R gamma, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-15Ra&IL-15, His Tag with an affinity constant of 0.084 nM as determined in SPR assay Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. 2-7 days, 2 ~8 °C under sterile conditions after reconstitution. 3 months, -25~-15℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Caution 1.Please operate with lab coats and disposable gloves,for your safety. 2.This product is for research use only. Show more