Specification Synonyms DR4; TRAIL-R; TNFRSF10A; TRAIL R1 Source Human TRAIL R1/DR4/TNFRSF10A Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Pro34-Asn239.[Accession | O00220] Molecular Weight The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity SPR Data: Human TRAIL R1, His Tag captured on CM5 Chip via anti-his antibody can bind Human TRAIL, No Tag with an affinity constant of 1.02 nM as determined in SPR assay . Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. 2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution. 3 -6 months, -85~-65℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Caution 1.Please operate with lab coats and disposable gloves,for your safety. 2.This product is for research use only.
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