Specifications Source Human CD44 Protein is expressed from HEK293 with His tag at the C-terminal. It contains Gln21-Pro220 & Gln386-Ala427.[Accession | P16070-1] Molecular Weight The protein has a predicted MW of 27.91 kDa. Due to glycosylation, the protein migrates to 45-68 kDa based on Tris-Bis PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 95% as determined by Tris-Bis PAGE and HPLC Activity ELISA Data: Immobilized Human CD44, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD44 Antibody, hFc Tag with the EC50 of 11.8ng/ml determined by ELISA. Formulation Supplied as 0.22μm filtered solution in PBS (pH 7.4). Storage The product should be stored at -85~-65℃ for 1 year from date of receipt. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Note 1.Please operate with lab coats and disposable gloves for your safety. 2.This product is for research use only.
